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Org. Biomol. Chem., 2009, 7, 5055 - 5058, DOI: 10.1039/b917119n

Clickable fluorescent dyes for multimodal bioorthogonal imaging

Lun K. Tsou, Mingzi M. Zhang and Howard C. Hang

Bioorthogonal ligation with functionalized fluorescent dyes enables visualization of nuclei acids, proteins and metabolites in biological systems. Bright and modular azide- and alkyne-functionalized dyes are therefore needed to expand the fluorescence imaging capabilities of bioorthogonal ligation methods. We describe the concise synthesis of clickable fluorescent dyes based on 2-dicyanomethylene-3-cyano-2,5-dihydrofuran fluorophores and demonstrate their utility for multicolor imaging of azide- and alkyne-modified proteins as well as FRET studies.

Graphical abstract image for this article (ID: b917119n)