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• Details of optical set-up, emulsion storage off-chip and description of the electro-coalescence device.
• (PDF format, KB)

• Movie M1 shows 2 pL IVT droplet production. The device contains a Ψ-shaped connection, in which two aqueous streams are merged and emulsified using 10 μm wide flow focusing junction. The fluorinated HFE-7500 oil used to produce droplets contained 3% (w/w) EA-surfactant. The droplets were collected at the exhaust.
• (MPG format, KB)

• Movie M2 shows IVT emulsion re-injection after 6 hours of incubation off-chip. The re-injection rate was 20 ěL/hr for the IVT emulsion and 180 μL/hr for the carrier oil used to space droplets. The carrier oil was FC40 with 3% (w/w) EA-surfactant.
• (MPG format, KB)

• Movie M3 shows droplet re-injection, pairing and electro-coalescence. 2 pL IVT droplets were reinjected at 20 μL/hr and spaced by carrier oil at 180 μL/hr. 10 pL Assay droplets were generated by the on-chip droplet generation module using 100 μL/hr for the aqueous phase and 200 μL/hr for the carrier oil. Carrier oil was FC40 with 3% (w/w) EA-surfactant. The fused droplets were collected at the exhaust.
• (MPG format, KB)

• Movie M4 shows the fusion module during electro-coalescence of 2 pL IVT droplets with 10 pL Assay droplets.
• (MPG format, KB)

• Movie M5 shows the fused droplets after electro-coalescence, collection off-chip and re-injection. Re-injection rate was 20 μL/hr for the emulsion and 120 μL/hr for the carrier oil used to space droplets.
• (MPG format, KB)

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