File Name : supplementary video 1 24mb.wmv

Caption : supplementary video 1. video showing experimental procedures for high-throughput, high-content histological analysis of 3d tumor spheroids by pillar array. the steps included are; assembly of the pillars in the pillar holder, loading and culturing cancer cells in 3d using 96-well plates, drug exposure, embedding spheroid arrays in the oct solution, sectioning the oct block, and conducting immunohistochemical staining.

File Name : supplementary video 2 1.7mb.wmv

Caption : supplementary video 2. a 3d reconstruction image of ht-29 spheroids following exposure to 100 µm of dox for 20 min. serial images were obtained by optical sectioning at 9 μm increments over 351 µm distance from the bottom to the center of an alginate cap along the z-axis; images of the region beyond this distance was not acquired due to optical attenuation. intranuclear accumulation of dox visualized by fluorescence imaging demonstrated 3d structure of ht-29 spheroids cultured in alginate matrix on pillar array. approximate sizes of spheroids appeared to be 113 ± 25 µm.