File Name : supplementary fig 1.tif

Caption : supplementary fig. 1: effects of different doses of troxerutin on the histopathological changes in the kidney tissues of the gentamycin-treated rats. representative photomicrographs from different experimental group: normal control group (n ctrl), troxerutin control group (tr ctrl), gentamycin-treated group (gn), gentamycin and 50 mg/kg troxerutin-treated group (gn+tr 50), gentamycin and 100 mg/kg troxerutin-treated group (gn+tr 100), gentamycin and 150 mg/kg troxerutin-treated group (gn+tr 150), and gentamycin and 200 mg/kg troxerutin-treated group (gn+tr 200). a representative photomicrographs from gn group (c) shows focal inflammatory cell infiltration in between dilated blood vessels and renal tubules (white arrow) compared to both n ctrl and the tr ctrl that show normal histological structure. a representative photomicrographs from different doses of gn+tr show gradually mitigated histopathological changes compared to gn group. red arrow indicates hemorrhage. the representative photomicrographs were taken from the kidney tissues one day after the last dose of gentamycin and stained with hematoxylin and eosin, magnification x 40. 

File Name : supplementary fig 2.tif

Caption : supplementary fig. 2: effects of different doses of troxerutin on glomerular filtration rate, and levels of serum creatinine and blood urea nitrogen in the gentamycin-treated rats.  glomerular filtration rate (a), level of serum creatinine (b), and level of blood urea nitrogen (c) were determined one day after the last dose of gentamycin in all of the experimental groups: normal control group (n ctrl), troxerutin control group (tr ctrl), gentamycin-treated group (gn), gentamycin and 50 mg/kg troxerutin-treated group (gn+tr 50), gentamycin and 100 mg/kg troxerutin-treated group (gn+tr 100), gentamycin and 150 mg/kg troxerutin-treated group (gn+tr 150), and gentamycin and 200 mg/kg troxerutin-treated group (gn+tr 200). data are presented as mean ± standard deviation, (*) significant difference from both n ctrl and tr ctrl groups, (#) significant difference from the gn group, p < 0.05 (n = 8). 

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Caption : supplementary fig. 3: effects of different doses of troxerutin on urinary albumin and urinary albumin to creatinine ratio in the gentamycin-treated rats.  urinary albumin (a)  and urinary albumin-to-creatinine ratio (b) were determined one day after the last dose of gentamycin in all of the experimental groups: normal control group (n ctrl), troxerutin control group (tr ctrl), gentamycin-treated group (gn), gentamycin and 50 mg/kg troxerutin-treated group (gn+tr 50), gentamycin and 100 mg/kg troxerutin-treated group (gn+tr 100), gentamycin and 150 mg/kg troxerutin-treated group (gn+tr 150), and gentamycin and 200 mg/kg troxerutin-treated group (gn+tr 200). data are presented as mean ± standard deviation, (*) significant difference from both n ctrl and tr ctrl groups, (#) significant difference from the gn group, p < 0.05 (n = 8). 

File Name : supplementary fig 4.tif

Caption : supplementary fig. 4: modulating effects of different doses of troxerutin on the oxidative tissue damage markers in the kidney tissues of the gentamycin-treated rats. the levels of malondialdehyde mda (a), protein carbonyl content pcc (b), reduced glutathione gsh (c), and the total antioxidant capacity tac (d) were determined one day after the last dose of gentamycin in different experimental groups: normal control group (n ctrl), troxerutin control group (tr ctrl), gentamycin-treated group (gn), gentamycin and 50 mg/kg troxerutin-treated group (gn+tr 50), gentamycin and 100 mg/kg troxerutin-treated group (gn+tr 100), gentamycin and 150 mg/kg troxerutin-treated group (gn+tr 150), and gentamycin and 200 mg/kg troxerutin-treated group (gn+tr 200). data are presented as mean ± standard deviation, (*) significant difference from both n ctrl and tr ctrl groups, (#) significant difference from the gn group, p < 0.05 (n = 8).