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Caption : fig. s1. histogram of particle-size distribution of dox-cl (a) and dox-sal (b) obtained by dynamic light scattering measurements. inset: the transmission electronic microscope image of dox-cl (a) and dox-sal (b). scale bar, 100 nm.

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Caption : fig. s2. the long-term stability and serum stability of liposomal dox. (a) particle size and (b) ee% changes of dox-load liposomes during 3 months storage at 4 °c. (a) particle size and (b) dox release% of dox-load liposomes after incubation at 37 °c in pbs (ph 7.4) with 10% fbs. data is shown as mean ± s.d. (n = 3). 

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Caption : fig. s3. (b) l-selectin expression on the membranes of pbns isolated from s180 tumor-bearing mice and normal mice. cells were stained with fitc-cd62l for l-selectin (green) and dapi for nuclei (blue), then observed by using a clsm. scale bar = 10 μm. (c) the mean fluorescence intensity of fitc-cd62l in pbns. **p < 0.01. data are expressed as mean ± s.d. (n = 3).

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Caption : fig. s4. cellular uptake of dox-cl and dox-sal by pbns. (a) quantity of dox in pbns after pbns (1 × 106 cells/ml) were incubated with dox-cl and dox-sal at different concentrations of dox for 30 min. (b) quantity of dox in pbns after pbns (1 × 106 cells/ml) were incubated with dox-cl and dox-sal (10 μg/ml) for different times. the data are presented as the mean ± s.d. (n = 3).

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Caption : fig. s5. (a) the expression level of cd11b on the cell membrane of pbns with internalized dox formulations in the absence or presence of fmlp (10 nm) for 1 h. cd11b was stained with fitc-conjugated cd11b antibody. (b) quantification of the cd11b expression on pbns. (c) superoxide anion generation of pbns with internalized dox formulations in the absence or presence of fmlp. superoxide anion were stained with dihydroethidium. (d) quantification of the superoxide anion generation by pbns. **p < 0.01. data are expressed as mean ± s.d. (n = 3).

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Caption : fig. s6. clsm images of s180 cells after incubation with pbns internalizing dir-labeled liposomes over time. the nuclei of cells were stained with hoechst 33342 (blue), the pbns were labelled with fitc-gr-1 (green) and the released dna segments were stained with pi (red). the merged image is the overlay of three individual images. the arrows indicate fibrous extracellular dna released by pbns after incubation with s180 tumor cells. scale bar, 10 µm.

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Caption : fig. s7. the classification of leukocytes and the dir-labeled liposomes uptake by pbns at different times using flow cytometry.

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Caption : fig. s8. percentage of pbns in peripheral blood leukocytes 24 h after i.p. injection of pbs (a) or 20 mg/kg of anti-gr-1 antibody (b). after the blood was treated with rbc lysis buffer, pbns were labelled by fitc-gr-1 antibody and analyzed using a fac sort flow cytometry. (n=3).

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Caption : fig. s9. quantification of the concentration of dir in collected tumors. at 24 h after i.p. injection of pbs or 20 mg/kg of anti-gr-1 antibody, mice were i.v. administered dir-labeled liposomes and the tumors were extracted from the mice at 1 h after injection. **p < 0.01. the data are expressed as the mean ± s.d. (n = 3). 

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Caption : fig. s10. pharmacokinetic behavior of different dox preparations in male kunming mice. the data are expressed as mean ± s.d (n = 3).