File Name : figure s1_ep timecourse.eps

Caption : primary human t cells were electroporated after being held in btx electroporation media for 0, 15, or 60 minutes. data is the average of replicates from 3 independent, healthy donors, and error bars represent the standard error of the mean.  (a) transfection efficiency, as measured by flow cytometry 24 hours after electroporation, is reduced when t cells are held in btxpress media with mrna for 15 min, and is reduced further if the hold time is increased to 60 min. (b) post-transfection viability, as measured by flow cytometry 24 hours after electroporation, decreases with increasing hold time, but the decrease is not statistically significant. 

File Name : figure s2 acoustics_modeling_figure_supplement.eps

Caption : (a)  acoustic pressure in a yz cross section of the channel at x = 10.5 mm with a focusing frequency of 933 khz. (b) for the same cross section, von mises stress and displacement with a scale factor of 5x107 are plotted demonstrating the coupling between the acoustic pressure in the channel and the distortion of the polystyrene walls.

File Name : figure s3_fluorescence mixing and sim.eps

Caption : acoustic stimulation does not disrupt the laminar sheath flow or cause mixing of the streams beyond diffusion. sodium fluorescein dissolved in pbs solution was flowed into the sheath streams at 60 µl/min, and btx solution was flowed into the center stream at 40 µl/min. (a) the fluorescence of fluid collected independently from the sheath and center outlets was measured using a fluorescence plate reader. (b) top-down views of the inlet and outlet regions of the device at positions indicated by the dotted box on the device schematic. images are shown with the acoustics off (black box), the acoustics on (red box), and for the simulated concentration distribution (grey box). for these images, sodium fluorescein in pbs solution was introduced into the sheath streams and btx solution was introduced into the center stream. the blue vertical lines in the images indicate the positions where we measured fluorescence intensity profiles across the channel width (at x = -17mm and x = +17mm). (c) normalized fluorescence intensity as a function of position across the width of the channel with acoustics turned off and on. position along the y-axis was normalized by the half-width of the channel. simulation data is also included for comparison.