File Name : figure_s1.tif

Caption : figure s1.  microfluidic dspe workflow and evaluation of automated closable valving using aqueous dyes. (a) workflow for dspe valving and washing protocol. colored dyes served as visual surrogates for each reaction component in the dspe protocol. green and white arrows represent valve opening and closures respectively. after dna binding and waste valve 1 opening (a-i), the crude cellular lysate (yellow) is spun to waste. the first waste valve is closed and the valve for the ipa wash is open (a-ii). ipa (red) is centrifugally pumped into the extraction chamber. the second waste valve is opened (a-iii). disc rotation forces ipa is to a waste chamber, the second waste valve is closed, and the te wash (green) valve is opened (a-iv). the te wash (green) is pumped into the extraction chamber and the third waste valve is opened (a-v). the third waste valve is closed and the valve between the te eluent (blue) and the extraction chamber is opened (a-vi). the buffer is released into the extraction chamber, the valve to the final elution chamber is opened, (a-vii) and the purified dna solution is isolated (a-viii). (b) the hue of the dye solutions were measured before (lighter traces) and after the dspe spin protocol (darker traces). the distribution of pixel count at each normalized hue value for each solution was plotted. for reference, a visual representation of the hue scale is given at the top of the graph. (c) initial and final hue values as well as the hue change are provided.

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Caption : figure s2.  calibration data showing the relationships between empirical colorimetric values and the log10 of erioglaucine dye concentration (n = 3). (a) hue values exhibited a roughly linear correlation with log10(conc.) with r2= 0.8928 and lodhue =  0.00195 mm. (b) saturation values exhibited a strong, nonlinear (sigmoidal) correlation with log10(conc.) with rss= 0.01181 and lodsaturation = 0.00391 mm. arrows indicate lods based upon colorimetric values for deionized water. [=  ̅_+(3∗ _ )]

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Caption : table s1. laser diode settings for all laser-based valving  experiments. (a) preliminary evaluation of the laser-based closure method. (b) disc material type and valve closure conditions for supplemental experiments that followed the preliminary statistical modelling (manual alignment of the target channel and laser diode).  (c) disc type and valve closure conditions for the optimization studies using the automated prtzal system. (d) valve opening and closure conditions for the dspe studies.

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Caption : table s2. logistic regression model main effect terms and interaction terms.

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Caption : table s3. rotational frequencies and spin times for the current dspe spin protocol.

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Caption : table s4. selected logistic regression models and the relevant associated values used for model evaluation and selection. (a) evaluation of the top logistic regression models (based on bic values) from preliminary valve closure experiments using the manual alignment system. (b) evaluation of the top logistic regression models from valve closure experiments using the automated prtzal system.

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Caption : table s5. success rates and confidence intervals for valve closure testing using the manual alignment system.

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Caption : table s6.  numerical image parameter values for hue and saturation as measured in contaminant removal study. hue and saturation were measured in an 8-bit colorspace. as such, original measurements were between 0 and 255. data were subsequently normalized through division by 256, resulting in a 0-1 scale. waste 1 contains the 1 mm erioglaucine solution originally loaded into the extraction chamber to simulate a crude cellular lysate from a buccal swab. waste 2, waste 3, and the final elution contain successive deionized water washes to remove residual contaminants from the extraction chamber. these values were then be compared to those of pure water measured in an on-disc microfluidic chamber. this testing was performed in triplicate and each value above represents a mean of three measurements taken from parallel assays.