File Name : supplementary 1.jpeg Caption : supplementary 1. lc50 values of treatments from different metal ions at 24 h and 96 h, intracellular cu content, relative expression of cu transporter genes in zfl cells. the curve was generated by non-linear regression. (a) cucl2, 24 h. (b) cucl2, 96 h. (c) zncl2, 24 h. (d) zncl2, 96 h. (e) the intracellular cu content of zfl after 24 h cucl2 exposure. the curve was generated by linear regression. (f) the relative expression of cu transporter genes related to zatp7b. the bars represent the geometric mean of fold difference derived from biological replicates (n = 3) and error bars represent 95% confidence level. t-test revealed a significant ∆ct difference (p >0.05) between groups a, b, and c. File Name : supplementary 2.jpeg Caption : supplementary 2. swiss-model-predicted cu transporter structures with domain map and plasmid map. (a) zctr1 (trimer, from 43th-184th aa). (b) zatox1 (dimer, from 1st-65th aa). (c) zatp7a (from 628st-1394th aa, without metal binding domain). (d) zatp7b (from 537st-1289th aa, without metal binding domain). (e) domain map of zctr1, zatox1, zatp7a and zatp7b. (f) plasmid map of p(cu transporter)-egfp, pegfp-(cu transporter) and pcdna3.1-(cu transporter). cmv: cytomegalovirus promoter used in mammalian expression. sv40: simian vacuolating virus 40 promoter used in mammalian expression. ampr: constitutive promoter used for expression in escherichia coli. neor/kanr: aminoglycoside phosphotransferase expression for kanamycin and g418 resistance. ori: origin of replication. File Name : supplementary 3.jpeg Caption : supplementary 3. protein sequence alignment of ctr1 and atox1 of different species by muscle. (a) zctr1: ctr1 from danio rerio (q8qh07). zctr1*: ctr1 from this study. sctr1: ctr1 from sparus aurata (q700f3). hctr1: ctr1 from homo sapiens (o15431). rctr1: ctr1 from rattus norvegicus (q9jk41). mctr1: ctr1 from mus musculus (q8k211). (b) zatox1: atox1 from danio rerio (e7fez9). zatox1*: atox1 from this study. satox1: atox1 from sparus aurata (q53hx5). hatox1: atox1 from homo sapiens (o00244). ratox1: atox1 from rattus norvegicus (q9wuc4). matox1: atox1 from mus musculus (o08997). File Name : supplementary 4.jpeg Caption : supplementary 4. protein sequence alignment of atp7a of different species by muscle. zatp7a: atp7a from danio rerio (q4f8h5). zatp7a*: atp7a from this study. satp7a: atp7a from sparus aurata (d4n236). hatp7a: atp7a from homo sapiens (q04656). ratp7a: atp7a from rattus norvegicus (p70705). matp7a: atp7a from mus musculus (q64430). File Name : supplementary 5.jpeg Caption : supplementary 5. protein sequence alignment of atp7b of different species by muscle. zatp7b: atp7b from danio rerio (i3itm6). zatp7b*: atp7b from this study. satp7b: atp7b from sparus aurata (d4n237). hatp7b: atp7b from homo sapiens (p35670). ratp7b: atp7b from rattus norvegicus (q64535). matp7b: atp7b from mus musculus (q64446). File Name : supplementary 6.jpeg Caption : supplementary 6. protein sequence alignment of zatp7a and zatp7b by muscle. zatp7a*: atp7a from this study. zatp7b*: atp7b from this study.