File Name : fig. s1.pdf
Caption : supplementary figure 1. t-dna insertion mutants of the cbc1 and cbc2 genes used in this study. (a) genomic structures of cbc1 (at3g01490) and cbc2 (at5g50000) and positions of t-dna insertions within an individual gene. boxes and bold lines indicate exons and introns, respectively. regions of coding sequence are shown as black boxes. (b) expression of cbc1 and cbc2 in wt and the cbc1cbc2-2 mutant. mrna expression was determined by reverse-transcription polymerase chain reaction (rt-pcr) using a specific primer set (table s1). tublin2 (tub2) mrna was detected as an internal control. (c) growth and morphology of phot1phot2, cbc1, cbc2-2, and cbc1cbc2-2. plants were grown on soil for 4 weeks and photographed. bar indicates 1 cm.
File Name : 2nd_revision_cbc_figs2.pdf
Caption : supplementary figure 2. amount of pm h+-atpase in guard cells of wt and cbc1cbc2-2. total pm h+-atpase proteins in guard cells were detected by the immunohistochemical method using anti-h+-atpase antibody as described previously26. isolation of epidermal fragments, blue light treatment, and quantification of the fluorescent signals were performed in the same ways as in fig. 3c. values are means ± sd (n = 3); 30 stomata were measured in each experiment. data are expressed relative to wt values. differences from wt were evaluated using student’s t test (n.s.= not significant).
File Name : 2nd_revision_cbc_figs3.pdf
Caption : supplementary figure 3. effect of allelic difference of cbc2 mutations on blue light-dependent stomatal responses in cbc1cbc2 double mutants. experiment was performed and shown as in figure 3a.