Additions and corrections
Studying Smad2 intranuclear diffusion dynamics by mathematical modelling of FRAP experiments
Vinicio González-Pérez, Bernhard Schmierer, Caroline S. Hill and Richard P. Sear
Integr. Biol., 2011, 3, 197–207 (DOI: 10.1039/C0IB00098A) Amendment published 25th March 2011
The following integration box was omitted from the article:
Insight, innovation, integration The dynamics of proteins in cells are crucial to their function, and changes in mobility reveal changes in protein state. Proteins are typically assumed to move via diffusion. Here we find that the signal transducer Smad2 appears never to move via simple diffusion. This calls into question current assumptions of protein dynamics. Modern confocal microscopes and advanced modelling need to be combined to test the hypothesis that a protein is diffusing. We present a novel semi-analytical analysis for photobleaching data. We show how modern microscopy and modelling need to be combined and that the resulting tool can rigorously test assumptions about the intracellular protein dynamics, as well as monitor changes in protein state during signalling. |
The Royal Society of Chemistry apologises for this error and any consequent inconvenience to authors and readers.
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