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Caption : Supplementary Figure 1. F-actin staining of compact hepatocyte aggregates at (A) day 1, (B) day 3, and (C) day 5 of culture.

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Caption : Supplementary Figure 2. Transcriptional activity of liver-specific genes in compact hepatocytes (circles) and 2D monolayer controls (squares) over 5 days of culture. Transcript levels were expressed relative to that of freshly isolated hepatocytes.  (A-D) Phase I metabolic genes, (E-H) Phase II metabolic genes, (I-L) Excretory transport genes, and (M-P) stress response genes. Data are average ± S.D. of 3 independent experiments.

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Caption : Supplementary Figure 3. Transcriptional profile changes of compact and non-compact hepatocytes after 7 days of culture. (A) β-actin normalized transcript levels relative to freshly isolated hepatocytes. Data are average ± min-max values of 3-4 channels. (B) Scatter plot comparing relative transcript levels of compact and non-compact hepatocytes as shown in (A). Dotted lines indicate 2-fold upregulation or downregulation.