File Name : supp_figs1.tif

Caption : figure s1. frequency of cell velocities (cell positions/h) along the crypt during index reassignment process, i.e. moving to the ring immediately above (blue columns), and during time intervals in which cells do not change rings (red columns). velocities are estimated in the three-dimensional space (a) as well as in each of the dimensions, z (b), x (c) and y (d). velocities were estimated from 14.000 cells located in the upper half of a simulated crypt during one week. numbers represent the average velocities and their standard errors for each group of cells according to colour.

File Name : supp_figs2.tif

Caption : figure s2. comparison of the theoretical velocity derived from the balance of forces within the crypt and the simulated velocity in our model. the difference between the theoretical and simulated velocity was measured in 40,000 cells evolving in a simulated crypt during one week. blue columns represent velocity frequencies for cells undertaking index reassignment and therefore moving to the ring immediately above, while red columns are for cells that do not change ring. numbers represent the average velocities for each group of cells according to colour.  

File Name : supp_figs3.tif

Caption : figure s3. longitudinal view of a simulated fission event. the white arrow marks the initial location of the bud in the primary crypt at the time of fission initiation. the bud grows into a crypt that migrates upwards the primary crypt in the following days.  the lumen (red) of both crypts is connected during this process.