Supplementary Material (ESI) for Molecular BioSystems
This journal is © The Royal Society of Chemistry 2009

The list of 61 different growth conditions, data of which is used to study the conditional essentilaity.

1:  20 ug of Total RNA from MG1655 (Repaired NCM 3416) in M9 at 30 degrees OD 600=0.8
2:  20 ug of Total RNA from MG1655 (Repaired NCM 3416) in LB at 30 degrees OD 600=0.8
3:  Wild Type (N3433)  M9 + 0.2% Glycerol, 0.2% Tryptone at 30 C Mid Log
4:  RhlB- (SU02) in   M9 + 0.2% Glycerol, 0.2% Tryptone at 30 C Mid Log
5:  Wild Type (K10)  M9 + 0.2% Glycerol, 0.2% Tryptone, 40 mM Suc. at 30 C Mid Log
6:  Eno- (DF261) in M9 + 0.2% Glycerol, 0.2% Tryptone, 40mM Suc. at 30 C Mid Log
7:  Pnp- (YHC012)  in  M9 + 0.2% Glycerol, 0.2% Tryptone at 30 C Mid Log
8:  WT (SH3208)  M9 + 0.2% Glycerol, 0.2% Tryptone at 30 C Mid Log
9:  N-RNaseE (BZ453)  in   M9 + 0.2% Glycerol, 0.2% Tryptone at 30 C Mid Log
10:  10min UV treated cells, 25 ug total RNA
11:  20min untreatedcells, 25 ug total RNA
12:  20min UV treated cells, 25 ug total RNA
13:  40min untreatedcells, 25 ug total RNA
14:  5min untreatedcells, 25 ug total RNA
15:  60min untreatedcells, 25 ug total RNA
16:  60min UV treated cells, 25 ug total RNA
17:  lexA 20 min after NOuv, 25 ug total RNA, 2 ug pdN6
18:  lexA 60 min after NOuv, 25 ug total RNA, 2 ug pdN6
19:  lexA 10 min after UV treatment, 25 ug total RNA, 2 ug pdN6
20:  lexA 20 min after UV treatment, 25 ug total RNA, 2 ug pdN6
21:  lexA 40 min after UV treatment, 25 ug total RNA, 2 ug pdN6
22:  lexA 5 min after UV treatment, 25 ug total RNA, 2 ug pdN6
23:  lexA 60 min after UV treatment, 25 ug total RNA, 2 ug pdN6
24:  0' lexA, before UV 25 ug total RNA, 2 ug pdN6
25:  5' in minimal medium +0.2% glu, 25 ug total RNA
26:  15' in minimal medium +0.2% glu, 25 ug total RNA
27:  30' in minimal medium +0.2% glu, 25 ug total RNA	
28:  60' in minimal medium +0.2% glu, 25 ug total RNA
29:  5' in min med +0.2% glu+50ug/ml Trp,25ug total RNA
30:  15' in min med +0.2% glu+50ug/ml Trp,25ug total RNA
31:  30' in min med +0.2% glu+50ug/ml Trp,25ug tot RNA
32:  60' in min med +0.2% glu+50ug/ml Trp,25ug tot RNA
33:  tnaA2trpR2, W3110 min +Trp 50ug/ml, 30 ug total
34:  W3110 trpR2,min+.2%glucose, 30 ug RNA
35:  trpR2+50ug/ml Trp, 30 ug total RNA
36:  W3110 trpR2+trp, 30 ug total RNA	
37:  trpEA2trpR2, W3110 min +Trp 50ug/ml, 30 ug total	
38:  tnaA2trpA46PR9, W3110 min , 30 ug total
39:  5' 10 ug/ml indole aa, minimal medium + 0.2% glu, 25 ug total
40:  15' 10 ug/ml indole aa, minimal medium + 0.2% glu, 25 ug total
41:  30' 10 ug/ml indole aa, minimal medium + 0.2% glu, 25 ug total
42:  60' 10 ug/ml indole aa, minimal medium + 0.2% glu, 25 ug total
43:  5' 15 ug/ml indole aa, minimal medium + 0.2% glu, 25 ug total
44:  15' 15 ug/ml indole aa, minimal medium + 0.2% glu, 25 ug total
45:  30' 15 ug/ml indole aa, minimal medium + 0.2% glu, 25 ug total RNA	
46:  60' 15 ug/ml indole aa, minimal medium + 0.2% glu, 25 ug total
47:  JCB570, OD600=0.5
48:  JQ004 (rraA deleted), OD600=0.5
49:  JCB570, OD600=1.0
50:  JQ004 (rraA deleted), OD600=1.0
51:  JCB570, OD600=0.3
52:  JQ004 (rraA deleted), OD600=0.3
53:  W3110 wt OD420~0.3 in MOPS  (ile, val, thi) + Leu, 20 ug total RNA, 10 ug pdN6
54:  W3110 lrp- at OD420~0.3 in MOPS  (ile, val, thi) + Leu, 20 ug total RNA ,10 ug pdN6
55:  W3110 wt in MOPS (ile, val, thi) at OD420~0.3, 20 ug total,10 ug pdN6
56:  W3110 lrp- at OD420~0.3 in MOPS  (ile, val, thi), 20 ug total, 10 ug pdN6
57:  WT1088 in LB at 37 Mid Log Phase
58:  dpiA 2x Overexpression pHI1429 in LB at 37 Mid Log Phase
59:  N3433 (wt) in LB at 30 degrees C
60:  N3433 (rne deletion) pBAD-RNE w/out Arabinose in LB at 30 degrees C
61:  N3433 (rng deletion) in LB at 30 degrees C