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{"sheet":"Table S1","index":0,"n_rows":25,"n_cols":12,"rows_kept":25,"cut":false,"hidden":false,"merged":[],"flags":["formulas_cached_only"]}
["This journal is © The Royal Society of Chemistry 2003"]
["Electronic Supplementary Information for article B210111B"]
["Quantitative Measurement of Male Steroid Hormones Using Automated On-line Solid Phase Extraction-Liquid Chromatography/"]
[null,"Tandem Mass Spectrometry and Comparison with Radioimmunoassay"]
[]
["Yu-Chen Changa*, Chien-Ming Lia, Lih-Ann Lia, Shiang-Bin Jongb, Pao-Chi Liaoc, and Louis W. Changa"]
[]
["a Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Taiwan and "]
["b Department of Medical Technology, Kaohsiung Medical University, Taiwan; and "]
["c Graduate Institute of Environmental and Occupational Health, College of Medicine, National Cheng-Kung University, Taiwan"]
[]
[]
["Table S1   On-line Solid-phase Extraction Procedure Recovery Rates of Target Analytes"]
[]
["Steroids","2 ng ml-1 ",null,null,null,"5 ng ml-1 ",null,null,null,"10 ng ml-1 "]
[null,"*Mean (%)","SD","RSD (%)",null,"*Mean (%)","SD","RSD (%)",null,"*Mean (%)","SD","RSD (%)"]
[]
["4-Androstene-3,17-dione",91.1,0.03,3.14,null,106.7,0.03,3.14,null,91.9,0.05,4.89]
[]
["Testosterone",91,0.02,2.21,null,100.2,0.03,3.25,null,91.6,0.03,3.4]
[]
["Dihydrotestosterone",91.2,0.13,14.48,null,111,0.11,9.53,null,86.6,0.05,6.15]
[]
[]
["* Mean = concentrations of each analyte determined with on-line SPE devided by concentrationis of each analyte determined without on-line SPE"]

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[]
[]
["Table S2   Matrix Effects on On-line Solid-phase Extraction Procedure Recovery of Target Analytes"]
[]
["Steroids","2 ng ml-1 ",null,null,null,"5 ng ml-1 ",null,null,null,"10 ng ml-1 "]
[null,"*Mean (%)","SD","RSD (%)",null,"*Mean (%)","SD","RSD (%)",null,"*Mean (%)","SD","RSD (%)"]
[]
["4-Androstene-3,17-dione",88.6,0.05,5.43,null,99.2,0.01,1.43,null,96.8,0.01,0.77]
[]
["Testosterone",89.5,0.04,4.71,null,103.1,0.01,1.05,null,101.6,0.01,1.02]
[]
["Dihydrotestosterone",83.3,0.08,9.4,null,88.1,0.06,6.25,null,96.7,0.09,8.84]
[]
[]
["*Mean = analyte concentrations in 50% cell culture medium in loading buffer divided by analyte concentrations in 100% loading buffer"]

{"sheet":"Table S3","index":2,"n_rows":28,"n_cols":5,"rows_kept":28,"cut":false,"hidden":false,"merged":[],"flags":["formulas_cached_only"]}
["Table S3   Intra-day Precision and Accuracy of Target Analytes in Cell Culture Medium Determined Using On-line SPE LC/MS/MS "]
[]
["Steroids",null,"Standard analytes levels (ng ml-1)"]
[null,null,2,5,10]
[]
["4-Androstene-3,17-dione","Mean (ng ml-1)",1.96,5.09,10.09]
[null,"SD (ng ml-1)",0.14,0.12,0.56]
[null,"*Precision (RSD, %)",7.38,2.39,5.59]
[null,"**Accuracy (RE, %)",-8.22,1.89,0.88]
[null,"N",9,9,9]
[]
[]
["Testosterone","Mean (ng ml-1)",2.06,5.4,10.6]
[null,"SD (ng ml-1)",0.16,0.2,0.52]
[null,"*Precision (RSD, %)",7.69,3.66,4.94]
[null,"**Accuracy (RE, %)",2.89,8.04,6.04]
[null,"N",9,9,9]
[]
[]
["Dihydrotestosterone","Mean (ng ml-1)",1.93,5.37,9.97]
[null,"SD (ng ml-1)",0.33,0.39,0.87]
[null,"*Precision (RSD, %)",17.33,7.32,8.68]
[null,"**Accuracy (RE, %)",-3.56,7.31,-0.27]
[null,"N",9,9,9]
[]
[]
["*Precision (RSD, %) = 100 x (standard deviation) / mean"]
["**Accuracy (RE, %) = 100 x (mean - added amount) / (added amount) "]

{"sheet":"Table S4","index":3,"n_rows":27,"n_cols":5,"rows_kept":27,"cut":false,"hidden":false,"merged":[],"flags":["formulas_cached_only"]}
["Table S4   Inter-day Precision and Accuracy of Target Analytes in Cell Culture Medium Determined Using On-line SPE LC/MS/MS "]
[]
[]
["Steroids",null,"Standard analytes levels (ng ml-1)"]
[null,null,2,5,10]
[]
["4-Androstene-3,17-dione","Mean (ng ml-1)",2.09,4.98,9.82]
[null,"SD (ng ml-1)",0.11,0.38,0.74]
[null,"*Precision (RSD, %)",5.48,7.63,7.5]
[null,"**Accuracy (RE, %)",4.33,-0.4,-1.8]
[null,"N",9,9,9]
[]
["Testosterone","Mean (ng ml-1)",2.06,5.17,10.18]
[null,"SD (ng ml-1)",0.1,0.34,0.71]
[null,"*Precision (RSD, %)",4.98,6.5,7]
[null,"**Accuracy (RE, %)",2.94,3.33,1.82]
[null,"N",9,9,9]
[]
["Dihydrotestosterone","Mean (ng ml-1)",2.45,5.1,9.14]
[null,"SD (ng ml-1)",0.31,0.64,1]
[null,"*Precision (RSD, %)",12.48,12.59,10.89]
[null,"**Accuracy (RE, %)",22.39,2,-8.61]
[null,"N",9,9,9]
[]
[]
["*Precision (RSD, %) = 100 x (standard deviation) / mean"]
["**Accuracy (RE, %) = 100 x (mean - added amount) / (added amount) "]