Additions and corrections, Analyst

Additions and corrections

Automatic extraction and processing of small RNAs on a multi-well/multi-channel (M&M) chip

Runtao Zhong, Kenneth Flack and Wenwan Zhong

Analyst, 2012, 137, 5546–5552 (DOI: 10.1039/C2AN35883B). Amendment published 30th November 2012.

The authors regret the following errors:

• On p. 5546 in the Abstract, the penultimate sentence should read:

It also permitted smooth integration with on-chip RT-PCR, which simultaneously detected the target microRNA, mir-191, expressed in fewer than 100 cancer cells.

• On p. 5551 the caption to Fig. 6 should read as follows:

Fig. 6 The integrated M&M chip-based SPE-RT-PCR for detection of miRNA expressed in cell lines. (A) The image of the entire chip obtained by the Typhoon gel scanner after on-chip RT-PCR; the grey spots corresponded to the wells in Zone 3 and 4 in Fig. 2; (B) plot of relative fluorescence unit (RFU) (spot intensity reported by ImageQuant) vs. log [copies of miRNA used in chip-PCR] (in control wells #5, 6, 7, and 8 in (A)). The arrows pointed to the intensities of well #2 and 4 in (A). Each concentration of standard dilution series was repeated once and error bars represent standard deviation.

• On p. 5551 in the Integrated analysis of miRNA expression in cells section the third sentence in the last paragraph should read:

The sensitivity turned out to be adequate for detection of the target miRNA from about 100 Jurkat or lower than 100 293T cells, estimated from dilution.

The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.

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