File Name : s1.tif

Caption : raw spectra of anticoagulants from extracted from blood vials (left) and analytical grade compound (right).


File Name : s2.tif

Caption : scores for dry (top) and wet (bottom) whole blood spectra collected in blood collection vials containing sc, edta or heparin. to the left of the scores are the corresponding pc1 (blue) and pc2 (red) loadings. clusters are less defined than in plasma spectra as rbcs dilute the amount of anticoagulant in contact with the crystal. 


File Name : s3.tif

Caption : scores for wet plasma spectra collected in vials containing sc (top), edta (middle) or heparin (bottom). to the left of the scores are the corresponding pc1 (blue) and pc2 (red) loadings. in all cases, there is little difference between 1 h and 4 h, however at 10 h there is a significant difference.  


File Name : s4.tif

Caption : scores plot (above) of rbc spectra isolated from blood collected in vials containing sc, edta or heparin. to the right of the scores are the corresponding pc1 (blue) and pc2 (red) loadings. clustering resembles pca on wet whole blood spectra.


File Name : s5.tif

Caption : calibration data for individual anticoagulants between 0-0.1 %. while sc (b) and edta (c) data sets do not form a linear regression, the heparin data set (d) has the potential to for a robust model at lower concentration ranges.