{"text_format":"2","workbook":"xls","n_sheets":4,"sheets":["general","Laccase-TEMPO-TA","ADH-TA","ADH-AmDH"],"not_rendered":{"charts":3,"images":0,"macros":0,"external_links":1,"defined_names":0,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"run_cost":"one","rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"general","index":0,"n_rows":10,"n_cols":14,"rows_kept":10,"cut":false,"hidden":false,"merged":[[5,3,5,5],[5,7,5,10],[5,12,5,14],[6,3,6,5],[6,12,6,14]],"flags":["formulas_cached_only"]}
["Environmental assessment of enzymatic selective amination protocols"]
[]
["General remarks:"]
["The E-factor was calculated for non-volatiles, gases and volatile compounds constituting final products. Solvents were treated separately. It was assumed the same isolation protocol"]
[]
[null,null,null,"E-factor (excl. solvents)",null,null,null,"Waste classification (excl. solvents)",{"$nulls":4},"E-factor (incl. solvents)"]
["method","yield [%]","by-products [%]","reaction",null,null,null,"inorg. salts","organics","by-products","others",null,"reaction"]
["Laccase/TEMPO and TA",91,"<3",null,8.3,null,null,6,1.6,0.5,0.2,null,null,274.3]
["ADH and TA",50,35,null,20.8,null,null,4.8,14.8,0.1,1.1,null,null,289.2]
["ADH and AmDH",96,"<3",null,56.8,null,null,55,1.5,0.2,0.1,null,null,494.5]

{"sheet":"Laccase-TEMPO-TA","index":1,"n_rows":26,"n_cols":8,"rows_kept":26,"cut":false,"hidden":false,"merged":[[0,0,0,8]],"flags":["formulas_cached_only"]}
["Laccase/TEMPO and TA"]
[]
[]
["alcohol 6a"]
["yield:",0.91]
[]
[]
["Material","(Non)volatile [mg]","(Non)volatile [E]","Solvent [mL]",null,null,"E factor:"]
["Starting Materials",{"$nulls":5},"reaction",274.33599999999996]
["substrate 6a",100]
["O2",0.8,0.023]
["laccase from T. versicolor",31,0.342]
["TEMPO",16,0.177]
["ATA-033",75,0.828]
["amine donor",64.6,0.24]
["PLP",3.7,0.041]
["citrate buffer",47.5,0.525]
["phosphate buffer",492,5.433]
["water",null,220.862,20]
["MTBE",null,40.86,5]
["acetonitrile",null,4.34,0.5]
[]
["By-Products"]
["Pyrrole",null,0.335]
["Water",null,0.18]
["Other",null,0.15]

{"sheet":"ADH-TA","index":2,"n_rows":24,"n_cols":8,"rows_kept":24,"cut":false,"hidden":false,"merged":[[0,0,0,8]],"flags":["formulas_cached_only"]}
["Alcohol dehydrogenase and TA"]
[]
[]
["4-phenylbutan-2-ol","taken from K. Tauber et al., Chem. Eur. J. 2013, 19, 4030-4035"]
["yield:",0.5]
[]
[]
["Material","(Non)volatile [mg]","(Non)volatile [E]","Solvent [mL]",null,null,"E factor:"]
["Starting Materials",{"$nulls":5},"reaction",289.19599999999997]
["alcohol substrate",7.5]
["ADH-A",0.5,0.134]
["ADH-007",10,2.684]
["AlaDH",1,0.268]
["BM-TA",20,5.369]
["NAD cofactor",0.7,0.188]
["PLP",0.2,0.054]
["L-alanine",22.3,5.986]
["Ammonium chloride",10.7,2.644]
["Phosphate buffer",8.2,2.201]
["water",null,268.426,1]
[]
["By-Products"]
["Water",null,0.121]
["Ketone intermediate + others",null,1.121]

{"sheet":"ADH-AmDH","index":3,"n_rows":21,"n_cols":8,"rows_kept":21,"cut":false,"hidden":false,"merged":[[0,0,0,8]],"flags":["formulas_cached_only"]}
["Alcohol dehydrogenase and amine dehydrogenase"]
[]
[]
["2-heptanol","taken from F. G. Mutti et al., Science 2015, 349, 1525-1529"]
["yield:",0.96]
[]
[]
["Material","(Non)volatile [mg]","(Non)volatile [E]","Solvent [mL]",null,null,"E factor:"]
["Starting Materials",{"$nulls":5},"reaction",494.52]
["alcohol",1.2]
["AA-ADH",0.5,0.438]
["LBADH",0.5,0.438]
["Ch1-AmDH",0.5,0.438]
["NAD cofactor",0.33,0.263]
["Ammonium formate",63.1,55.001]
["water",null,437.738,0.5]
[]
[]
["By-Products"]
["water",null,0.156]
["Others",null,0.048]