File Name : table s1 wang.pdf

Caption : table s1: amount of reagents needed for the synthesis of methacrylated ecm molecules. nhs: n-hydroxysuccinimide. edc: 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. aema: 2-aminoethyl methacrylate

File Name : fig s1 wang.pdf

Caption : figure s1: neonatal chondrocyte (nchon) viability assayed via the live/dead kit on day 1 and day 14 after encapsulation in 11% pegdma hydrogels. ecm molecules were added to the hydrogels as indicated. scale bar, 400 μm.

File Name : fig s2 wang.pdf

Caption : figure s2: effects of mechanical stiffness and type of ecm on collagen x secretion, as shown by immunostaining. only hydrogels containing 5% (w/v) cs-ma, ha-ma, or hs-ma are shown. note that the green channel was color-boosted five-fold versus the staining for collagen i and collagen ii depicted in figures 4 and 5 of the main text. scale bars, 200 μm.

File Name : wang.figs3.tif

Caption : figure s3: swelling ratio of combinatorial hydrogels containing varying concentration of pegdma (8%, 11% and 14%) (w/v), varying ecm types (cs-ma, ha-ma and hs-ma), and varying ecm concentrations (0.5%, 1.25%, 2.5%, 5%). pegdma hydrogels that do not contain any ecm were included as controls. 

File Name : wang.figs4.tif

Caption : figure s4: negative immunostaining controls for type ii collagen (a) aggrecan (b) type i collagen (c) and type x collagen (d) as shown by immunostaining. blue: dapi. (scale bar = 200um)