{"text_format":"2","workbook":"xls","n_sheets":3,"sheets":["animal data","MS data males","pathway analysis"],"not_rendered":{"charts":0,"images":0,"macros":0,"external_links":1,"defined_names":0,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"run_cost":"one","rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"animal data","index":0,"n_rows":37,"n_cols":16,"rows_kept":37,"cut":false,"hidden":false,"merged":[[26,6,27,6],[26,7,27,7],[14,5,15,5],[24,5,25,5],[20,5,21,5],[14,7,15,7],[18,5,19,5],[16,7,17,7],[18,6,19,6],[18,7,19,7],[30,0,31,0],[20,0,21,0],[28,0,29,0],[30,5,31,5],[30,6,31,6],[20,7,21,7],[26,5,27,5],[24,0,25,0],[24,6,25,6],[24,7,25,7],[6,0,7,0],[8,0,9,0],[10,0,11,0],[14,0,15,0],[16,0,17,0],[18,0,19,0],[12,0,13,0],[4,9,4,15],[6,12,7,12],[30,7,31,7],[28,7,29,7],[28,5,29,5],[28,6,29,6],[12,5,13,5],[8,5,9,5],[8,6,9,6],[8,7,9,7],[6,6,7,6],[6,7,7,7],[10,5,11,5],[20,6,21,6],[4,2,4,8],[6,5,7,5],[12,6,13,6],[12,7,13,7],[16,5,17,5],[16,6,17,6],[6,13,7,13],[6,14,7,14],[8,12,9,12],[8,13,9,13],[12,12,13,12],[12,13,13,13],[12,14,13,14],[8,14,9,14],[10,14,11,14],[10,6,11,6],[10,7,11,7],[10,12,11,12],[10,13,11,13],[14,12,15,12],[14,13,15,13],[14,6,15,6],[14,14,15,14],[16,12,17,12],[16,13,17,13],[16,14,17,14],[18,12,19,12],[18,13,19,13],[18,14,19,14],[20,12,21,12],[20,13,21,13],[20,14,21,14],[24,12,25,12],[24,13,25,13],[24,14,25,14],[22,14,23,14],[22,0,23,0],[22,12,23,12],[22,13,23,13],[22,5,23,5],[22,6,23,6],[22,7,23,7],[30,12,31,12],[30,13,31,13],[30,14,31,14],[26,0,27,0],[26,12,27,12],[26,13,27,13],[26,14,27,14],[28,12,29,12],[28,13,29,13],[28,14,29,14]],"flags":["formulas_cached_only"]}
["Suppl. Table 1"]
["Animal data (both genders)"]
["Animals were exposed to 0, 3, or 30 mg/kg bw/d for 7 days"]
[]
[null,null,"Females",{"$nulls":6},"Males"]
[null,"HBCD-group",0,3,30,"Thyroid effect (P-value)","HBCD effect (P-value)","Interaction (P-value)","Spearman's Rho",0,3,30,"Thyroid effect (P-value)","HBCD effect (P-value)","Interaction (P-value)","Spearman's Rho"]
["Body weight (g)","ET","267 ± 19","261 ± 22","276 ± 24","<0.001","0.768"," 0.540","0.162","427 ± 36","428 ± 35","430 ± 27","<0.001","0.828","0.736","-0.045"]
[null,"HT","143 ± 37","136 ± 22","132 ± 25",null,null,null,"-0.010","165 ± 22","153 ± 45","146 ± 32",null,null,null,"-0.272"]
["Liver weight (g)","ET","9.50 ± 1.07","8.95 ± 1.39","9.63 ± 1.15","<0.001","0.452","0.839","-0.048","16.30 ± 1.12","16.14 ± 1.93","16.44 ± 1.68","<0.001","0.867","0.766","0.058"]
[null,"HT","4.56 ± 1.49","4.02 ± 0.71","4.23 ± 0.71",null,null,null,"-0.059","5.49 ± 0.80","5.12 ± 1.71","4.78 ± 1.11",null,null,null,"-0.295"]
["TSH (ng/ml)","ET","0.33 ± 0.08","0.42 ± 0.10","0.48 ± 0.15","<0.001","0.553","0.528","0.719*","0.38 ± 0.34","1.27 ± 0.92","0.49 ± 0.27","<0.001","0.810","0.409","0.168"]
[null,"HT","14.68 ± 10.25","16.36 ± 6.37","11.86 ± 1.62",null,null,null,"-0.259","17.01 ± 5.06","14.51 ± 4.31","16.76 ± 4.40",null,null,null,"-0.012"]
[" total T4 (µg/dl)","ET","n.a.","n.a.","n.a.",{"$nulls":4},"4.4 ± 0.3","3.9 ± 0.8","4.2 ± 0.5","<0.001","0.189","0.229","-0.306"]
[null,"HT","n.a.","n.a.","n.a.",{"$nulls":4},"<lod","<lod","<lod",null,null,null,"0.433"]
[" total T3 (ng/ml)","ET","362 ± 79","369 ± 60","305 ± 38","0.003","0.177","0.385","-0.200","230 ± 48","234 ± 41","196 ± 9","<0.001","0.189","0.587","-0.35"]
[null,"HT","305 ± 18","259 ± 90","265 ± 53",null,null,null,"-0.343","165 ± 12","177 ± 25","161 ± 47",null,null,null,"-0.148"]
["free T3 (pg/ml)","ET","6.62 ± 1.10","7.30 ± 0.80","6.56 ± 0.87","<0.001","0.478","0.460","-0.061","5.95 ± 0.51","6.30 ± 0.61","5.27 ± 0.87","<0.001","0.044","0.326","-0.341"]
[null,"HT","1.41 ± 0.80","1.66 ± 0.20","1.91 ± 0.97",null,null,null,"0.214","1.56 ± 0.31","2.39 ± 0.78","1.80 ± 0.60",null,null,null,"0.041"]
["LH (ng/ml)","ET","3.64 ± 1.19","1.72 ± 0.84","2.49 ± 0.75","0.487","0.042","0.795","-0.346","0.61 ± 0.15","1.22 ± 0.51","0.88 ± 0.30","0.002","0.021","0.088","0.33"]
[null,"HT","3.09 ± 1.71","1.91 ± 1.32","2.81 ± 1.46",null,null,null,"-0.060","0.54 ± 0.18","0.62 ± 0.28","0.55 ± 0.13",null,null,null,"0.018"]
["FSH (ng/ml)","ET","2.8 ± 1.8","3.7 ± 2.9","3.6 ± 2.4","0.487","0.414","0.432","0.043","3.81 ± 0.43","5.04 ± 0.80","4.00 ± 0.83","0.065","0.039","0.491","0.161"]
[null,"HT","3.8 ± 1.08","2.8 ± 0.8","5.2 ± 3.6",null,null,null,"0.000","3.72 ± 1.15","4.16 ± 1.75","2.91 ± 0.83",null,null,null,"-0.359"]
["Testosterone (ng/ml)","ET","n.a.","n.a.","n.a.",{"$nulls":4},"2.23 ± 1.93","1.09 ± 0.36","1.24 ± 0.42","0.645","0.751","0.217","-0.308"]
[null,"HT","n.a.","n.a.","n.a.",{"$nulls":4},"0.98 ± 0.65","1.35 ± 1.45","1.64 ± 1.54",null,null,null,"0.233"]
["Leptin (ng/ml)","ET","5 ± 2","5 ± 1","5 ± 2","<0.001","0.445","0.487","-0.063","8 ± 2","7 ± 2","8 ± 2","0.006","0.205","0.134","0.022"]
[null,"HT","19 ± 9","22 ± 15","14 ± 8",null,null,null,"-0.364","13 ± 4","12 ± 4","8 ± 4",null,null,null,"-0.428"]
["Insulin (ng/ml)","ET","n.a.","n.a.","n.a.",{"$nulls":4},"6.02 ± 1.92","5.18 ± 2.69","8.81 ± 3.77","<0.001","0.174","0.035","0.261"]
[null,"HT","n.a.","n.a.","n.a.",{"$nulls":4},"4.38 ± 2.08","2.26 ± 1.48","2.09 ± 0.81",null,null,null,"-0.613**"]
["Corticosterone (ng/ml)","ET","281 ± 169","413 ± 292","338 ± 179","0.090","0.904","0.497","0.101","149 ± 71","103 ± 62","222 ± 69","0.027","0.056","0.549","0.35"]
[null,"HT","249 ± 115","187 ± 201","242 ± 171",null,null,null,"-0.007","183 ± 46","242 ± 113","353 ± 260",null,null,null,"0.193"]
["internal g-HBCD (mg/kg lw) ","ET","<lod","56.8 ± 17.7","113.2 ± 24.2",0.092,"<0.001",0.016,null,"<lod","25.5 ± 5.2","81.0 ± 33.1","0.002","<0.001","0.013"]
[null,"HT","<lod","50.1 ± 13.5","160.0 ± 46.0",{"$nulls":4},"<lod","40.7 ± 8.6","130.0 ± 32.9"]
[]
["<lod, below detection limit"]
["n=6 per group. Mean ± SD is given."]
["lw, lipid weight"]
["n.a., not analysed"]

{"sheet":"MS data males","index":1,"n_rows":22,"n_cols":9,"rows_kept":22,"cut":false,"hidden":false,"merged":[[6,7,7,7],[6,8,7,8],[6,1,7,1],[6,2,7,2],[6,3,7,3],[6,4,7,4],[6,5,7,5],[6,6,7,6]],"flags":["formulas_cached_only"]}
["Suppl. Table 2: "]
["MALDI TOF/TOF data of identified protein spots regulated in concentration in male rats. "]
["Numbering as in Figure 1. For regulation data see Table 2, complete peptide list is to be found in (Miller et al., submitted). Abbreviations: Protein Id (protein identification, from UniProt or NCBI database), Cov. % (sequence coverage)."]
[]
[]
[]
[null,"Protein Name","Protein Id","Mowse score","Cov. %","N° of queries matched","P-value","Theor. pI","Theor. Mr"]
["Spot N°"]
[1105,"Aldehyde dehydrogenase family1 memberL1","AL1L1_RAT",445,0.22,21,"2.40E-41","5.79",99976]
[1107,"Glycogen phosphorylase, liver form ","PYGL_RAT",111,0.1,11,"6.10E-08","6.75",97877]
[1165,"Heat shock cognate 71kDa protein","HSP7C_RAT",443,0.41,30,"3.80E-41","5.37",71055]
[1226,"Formimidoyltransferase-cyclodeaminase","FTCD_RAT",411,0.43,25,"6.10E-38","5.80",59504]
[1271,"Succinate-semialdehyde dehydrogenase, mitochondrial","SSDH_RAT",77,0.13,7,"0.00015","8.35",56723]
[1289,"Keratin, type I cytoskeletal 18 ","K1C18_RAT",300,0.41,19,"7.70E-27","5.17",47732]
[1349,"Cystathionine gamma-lyase ","CGL_RAT",407,0.4,17,"1.50E-37","8.20",44262]
[1357,"Serum paraoxonase/arylesterase2","PON2_RAT",145,0.12,7,"2.40E-11","5.30",39764]
[1358,"Cystathionine gamma-lyase ","CGL_RAT","365  ",0.37,17,"2.40E-33","8.20",44262]
[1376,"Serum paraoxonase/arylesterase 1 ","PON1_RAT","208 ",0.13,5,"1.20E-17","5.06",39561]
[1413,"Small glutamine-rich tetratricopeptide repeat-containing protein alpha ","SGTA_RAT",62,0.08,3,"0.0048","5.05",34307]
[1502,"Guanidinoacetate N-methyltransferase ","GAMT_RAT",179,0.39,15,"9.60E-15","5.69",26675]
[1554,"Cytochrome b5 ","CYB5_RAT",127,0.2,5,"1.50E-09","4.90",15346]
[1559,"Calmodulin ","CALM_RAT",135,0.1,2,"2.40E-10","4.09",16827]

{"sheet":"pathway analysis","index":2,"n_rows":91,"n_cols":11,"rows_kept":91,"cut":false,"hidden":false,"merged":[],"flags":["formulas_cached_only"]}
["Suppl. Table 3"]
["Significant changes between male and female rats (controls)"]
[]
[]
["STRING classification / KEGG pathways"]
[null,null,"Gender differences in ET animals (KEGG)",{"$nulls":4},"Gender differences in HT animals (KEGG)"]
[null,null,"(P<0.05)",{"$nulls":4},"(P<0.05)"]
[]
[null,null,"GO_id","Term","Number of Genes/Proteins","p-value",null,"GO_id","Term","Number of Genes/Proteins","p-value"]
[null,null,"rno01100","Metabolic pathways",22,"1.29E-13",null,"rno01100","Metabolic pathways",44,"3.2E-32"]
[null,null,"rno00980","Metabolism of xenobiotics by cytochrome P450",8,"7.8E-12",null,"rno00980","Metabolism of xenobiotics by cytochrome P450",3,"2.3E-3"]
[null,null,"rno00982","Drug metabolism - cytochrome P450",8,"3.16E-11",null,"rno00982","Drug metabolism - cytochrome P450",3,"3.71E-3"]
[null,null,"rno00480","Glutathione metabolism",8,"9.88E-13",null,"rno00480","Glutathione metabolism",3,"1.13E-3"]
[null,null,"rno00561","Glycerolipid metabolism",2,"8.35E-3",null,"rno00561","Glycerolipid metabolism",2,"1.79E-2"]
[null,null,"rno00010","Glycolysis / Gluconeogenesis",4,"2.03E-5",null,"rno00010","Glycolysis / Gluconeogenesis",6,"1.44E-7"]
[null,null,"rno00071","Fatty acid metabolism",4,"8.26E-6",null,"rno00071","Fatty acid metabolism",7,"8.12E-10"]
[null,null,"rno04146","Peroxisome",4,"6.22E-5",null,"rno04146","Peroxisome",5,"1.69E-5"]
[null,null,"rno04141","Protein processing in endoplasmic reticulum",3,"9.85E-3",null,"rno04141","Protein processing in endoplasmic reticulum",7,"4.47E-6"]
[null,null,"rno00053","Ascorbate and aldarate metabolism",3,"1.88E-5",null,"rno00053","Ascorbate and aldarate metabolism",4,"9.93E-7"]
[null,null,"rno00270","Cysteine and methionine metabolism",3,"1.11E-4",null,"rno00270","Cysteine and methionine metabolism",3,"3.64E-4"]
[null,null,"rno00330","Arginine and proline metabolism",3,"4.23E-4",null,"rno00330","Arginine and proline metabolism",7,"2.07E-9"]
[null,null,"rno00380","Tryptophan metabolism",3,"2.52E-4",null,"rno00380","Tryptophan metabolism",5,"1.07E-6"]
[null,null,"rno00340","Histidine metabolism",2,"2.23E-3",null,"rno00340","Histidine metabolism",2,"4.88E-3"]
[null,null,"rno00280","Valine, leucine and isoleucine degradation",2,"8.01E-3",null,"rno00280","Valine, leucine and isoleucine degradation",6,"4.8E-8"]
[null,null,"rno00310","Lysine degradation",2,"8.69E-3",null,"rno00310","Lysine degradation",5,"2.1E-6"]
[null,null,"rno00260","Glycine, serine and threonine metabolism",2,"3.24E-3",null,"rno00260","Glycine, serine and threonine metabolism",5,"1.56E-7"]
[null,null,"rno00410","beta-Alanine metabolism",2,"2.23E-3",null,"rno00410","beta-Alanine metabolism",2,"4.88E-3"]
[null,null,"rno00020","Citrate cycle (TCA cycle)",2,"3.24E-3",null,"rno00020","Citrate cycle (TCA cycle)",5,"1.56E-7"]
[null,null,"rno00030","Pentose phosphate pathway",2,"2.41E-3",null,"rno00030","Pentose phosphate pathway",3,"1.72E-4"]
[null,null,"rno00040","Pentose and glucuronate interconversions",2,"1.7E-3",null,"rno00040","Pentose and glucuronate interconversions",3,"1.01E-4"]
[null,null,"rno00620","Pyruvate metabolism",2,"4.7E-3",null,"rno00620","Pyruvate metabolism",5,"4.17E-7"]
[null,null,"rno00640","Propanoate metabolism",2,"3.7E-3",null,"rno00640","Propanoate metabolism",5,"2.21E-7"]
[null,null,"rno00650","Butanoate metabolism",2,"2.61E-3",null,"rno00650","Butanoate metabolism",2,"5.71E-3"]
[null,null,"rno03320","PPAR signaling pathway",2,"1.83E-2",null,"rno03320","PPAR signaling pathway",4,"2.64E-4"]
[null,null,"rno04612","Antigen processing and presentation",2,"1.93E-2",null,"rno04612","Antigen processing and presentation",3,"4.01E-3"]
[{"$nulls":7},"rno04145","Phagosome",5,"5.43E-4"]
[{"$nulls":7},"rno00051","Fructose and mannose metabolism",3,"3.64E-4"]
[{"$nulls":7},"rno00250","Alanine, aspartate and glutamate metabolism",3,0.0003]
[{"$nulls":7},"rno00350","Tyrosine metabolism",3,0.0003]
[{"$nulls":7},"rno00630","Glyoxylate and dicarboxylate metabolism",3,"3.52E-5"]
[{"$nulls":7},"rno00910","Nitrogen metabolism",3,"1.17E-4"]
[{"$nulls":7},"rno04540","Gap junction",3,"5.36E-3"]
[{"$nulls":7},"rno04910","Insulin signaling pathway",3,"1.63E-2"]
[{"$nulls":7},"rno04978","Mineral absorption",3,"8.16E-4"]
[{"$nulls":7},"rno05016","Huntington's disease",3,"4.36E-2"]
[{"$nulls":7},"rno00140","Steroid hormone biosynthesis",2,"1.38E-2"]
[{"$nulls":7},"rno00360","Phenylalanine metabolism",2,"1.9E-3"]
[{"$nulls":7},"rno00500","Starch and sucrose metabolism",2,"1.02E-2"]
[{"$nulls":7},"rno00860","Porphyrin and chlorophyll metabolism",2,"7.56E-3"]
[{"$nulls":7},"rno01040","Biosynthesis of unsaturated fatty acids",2,"4.88E-3"]
[null,null,"rno05215","Prostate cancer",2,"2.57E-2"]
[]
[]
[]
[null,null,"rno00480","Glutathione metabolism",null,"f/m",null,"rno00480","Glutathione metabolism"]
["lower",null,"Gsta4","Glutathione S-transferase alpha-4","GSTA4_RAT",null,null,"Gsta4","Glutathione S-transferase alpha-4","GSTA4_RAT"]
["higher",null,"Yc2","Glutathione S-transferase alpha-5","GSTA5_RAT",null,null,"Gstm7","Glutathione S-transferase Yb-3","GSTM4_RAT"]
["affected in ET and HT",null,"Gsta2","Glutathione S-transferase alpha-1","GSTA1_RAT",null,null,"Gss","Glutathione synthetase","GSHB_RAT"]
[null,null,"Gstp1","Glutathione S-transferase P","GSTP1_RAT"]
[null,null,"Gsta3","Glutathione S-transferase alpha-3","GSTA3_RAT"]
[null,null,"LOC494499","Glutathione S-transferase alpha-2","GSTA2_RAT"]
[null,null,"Gstm2","Glutathione S-transferase Mu 2","GSTM2_RAT"]
[null,null,"Idh1","Isocitrate dehydrogenase NADP cytoplasmic","IDHC_RAT"]
[]
[null,null,"rno00980","Metabolism of xenobiotics by cytochrome P450",null,null,null,"rno00980","Metabolism of xenobiotics by cytochrome P450"]
[null,null,"rno00982","Drug metabolism - cytochrome P450",null,null,null,"rno00982","Drug metabolism - cytochrome P450"]
[null,null,"Gsta4","Glutathione S-transferase alpha-4","GSTA4_RAT",null,null,"Gsta4","Glutathione S-transferase alpha-4","GSTA4_RAT"]
[null,null,"Adh4","Alcohol dehydrogenase class-3","ADHX_RAT",null,null,"Adh4","Alcohol dehydrogenase class-3","ADHX_RAT"]
[null,null,"Gstp1","Glutathione S-transferase P","GSTP1_RAT",null,null,"Gstm7","Glutathione S-transferase Yb-3","GSTM4_RAT"]
[null,null,"LOC494499","Glutathione S-transferase alpha-2","GSTA2_RAT"]
[null,null,"Gstm2","Glutathione S-transferase Mu 2","GSTM2_RAT"]
[null,null,"Yc2","Glutathione S-transferase alpha-5","GSTA5_RAT"]
[null,null,"Gsta2","Glutathione S-transferase alpha-1","GSTA1_RAT"]
[null,null,"Gsta3","Glutathione S-transferase alpha-3","GSTA3_RAT"]
[]
[null,null,"rno00071","Fatty acid metabolism",null,null,null,"rno00071","Fatty acid metabolism"]
[null,null,"Aldh1b1","Aldehyde dehydrogenase X_mitochondrial","AL1B1_RAT",null,null,"Aldh1b1","Aldehyde dehydrogenase X_mitochondrial","AL1B1_RAT"]
[null,null,"Acox1","Peroxisomal acyl-coenzyme A oxidase 1","ACOX1_RAT",null,null,"Acox1","Peroxisomal acyl-coenzyme A oxidase 1","ACOX1_RAT"]
[null,null,"Adh4","Alcohol dehydrogenase class-3","ADHX_RAT",null,null,"Adh4","Alcohol dehydrogenase class-3","ADHX_RAT"]
[null,null,"Aldh2","Aldehyde dehydrogenase_mitochondrial","ALDH2_RAT",null,null,"Aldh2","Aldehyde dehydrogenase_mitochondrial","ALDH2_RAT"]
[{"$nulls":7},"Acat1","Acetyl-CoA acetyltransferase_mitochondrial","THIL_RAT"]
[{"$nulls":7},"Acsl1","Long-chain-fatty-acid--CoA ligase 1","ACSL1_RAT"]
[{"$nulls":7},"Acaa1","3-ketoacyl-CoA thiolase A_peroxisomal","THIKA_RAT"]
[]
[null,null,"rno00010","Glycolysis / Gluconeogenesis",null,null,null,"rno00010","Glycolysis / Gluconeogenesis"]
[null,null,"Aldob","Fructose-bisphosphate aldolase B","ALDOB_RAT",null,null,"Aldob","Fructose-bisphosphate aldolase B","ALDOB_RAT"]
[null,null,"Adh4","Alcohol dehydrogenase class-3","ADHX_RAT",null,null,"Adh4","Alcohol dehydrogenase class-3","ADHX_RAT"]
[null,null,"Aldh2","Aldehyde dehydrogenase_mitochondrial","ALDH2_RAT",null,null,"Aldh2","Aldehyde dehydrogenase_mitochondrial","ALDH2_RAT"]
[null,null,"Aldh1b1","Aldehyde dehydrogenase X_mitochondrial","AL1B1_RAT",null,null,"Aldh1b1","Aldehyde dehydrogenase X_mitochondrial","AL1B1_RAT"]
[{"$nulls":7},"Fbp1","Fructose-1_6-bisphosphatase 1","F16P1_RAT"]
[{"$nulls":7},"Pklr","Pyruvate kinase isozymes R/L","KPYR_RAT"]