File Name : fig 1s.tif Caption : figure 1b absorption spectra of thionine in dmf (1.5 × 10-5 m, d = 10 mm) titrated with increasing volumes of aqueous solution of hydrochloric acid (5 × 10-3 m). arrows indicate changes induced by increasing concentration of the acid. File Name : fig 2s.tif Caption : figure 2s fluorescence spectra of thionine in dmf (2.2 × 10-6 m, 0.6 ml) with addition of 10 µl of aqueous solution of naoh (0.1 m). asterix denotes scattered excitation light reaching to the detector. File Name : fig 3s.tif Caption : figure 3s fluorescence spectra of thionine in dmf (2.2 × 10-6 m) registered with lower excitation wavelengths. signals from the scattered excitation light were removed for clarity. varying intensities are related to various absorbances values at the excitation wavelength. File Name : fig 4s.tif Caption : figure 4s transient absorption decays in dmf registered for the thionine (5 × 10-5 m , black curve) and th-go (0.5 mg/ml, red curve) following the 640 nm laser excitation. File Name : fig 5s.tif Caption : figure 5s a) transient absorption spectra registered at various time delays for thionine (5 × 10-5 m) in dmf following the 640 nm laser excitation. b) transient absorption kinetics registered at various wavelengths. File Name : fig 6s.tif Caption : figure 6s transient absorption kinetics registered at various wavelengths for a) thionine (5 × 10-5 m) and b) th-go (0.5 mg/ml) in dmf following the 450 nm laser excitation.