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Caption : schematic of the experimental set-up. pbs1-2: polarizing beam splitters, cl1-2: cylindrical lenses, m1-7: mirrors, sm: switching mirror: switches the collected light between bright field imaging ccd and raman spectrograph, obj1: for focusing the 1064 nm laser on to the sample plane and collecting the raman spectra; obj2: for focusing the raman excitation beam on to the sample, p1-2: polarizers, hwp1-2: half wave plates for power adjustment for raman excitation beam and trap beam respectively, hwp3 and hwp4: half wave plates for power adjustment in the two line optical tweezers, nf: notch filter, dm1: reflects the 514.5 nm laser and transmits rest of light from the illuminating source; tl: tube lens, cml: compensatory lens for collimating the emission signal, dm2: 1064 nm laser line mirror, l1-2, l5-6: lens pair as beam expander, l3: coupling lens to complete 4f system, l4: lens coupling the raman scattered light into the spectrograph. schematic expanded view of the two line tweezers (black) at the sample plane guiding the cells (red) is shown in the inset.
File Name : figure 2s.tif
Caption : pdms based microfluidic chip connected with inlets and outlets for raman flow cell cytometer, scale bar: 2.7 mm
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Caption : raw spectrum of a rbc taken while it was passing through the raman excitation beam in the microfluidic chip.
File Name : figure 4s.tif
Caption : (a) schematics of the forces acting on a particle while the particle interacts with line optical tweezers ‘1’ and ‘2’ in fluid flow. ft1 and ft2 are transverse component of optical forces for line tweezers ‘1’ and ‘2’, fd: drag force, θ: tilt angle for line tweezers w.r.t fluid flow direction (b) the transverse component of optical forces due to line tweezers and the drag force on the red blood cell, lt: line tweezers.
File Name : figure 5s.tif
Caption : (a) the image of the dual line optical tweezers arranged in the shape “υ”, green colour spot is the raman excitation, the line tweezers have been labelled as ‘1’ and ‘2’. scale bar: 10 μm (b) a snapshot from the video showing cells getting guided to the tail of the optical guide arrangement (multimedia view). the semi transparent red region schematically shows the line profile of the dual line optical tweezers.
File Name : figure 6s.tif
Caption : the average raman spectra of normal-rbc (blue) and met-rbc (red) taken over 25 cells. the spectra shown are area normalized to emphasize the relative changes in the intensities of various bands.
File Name : figure 7s.tif
Caption : the area normalized average spectra of (a) normal-rbc (blue) (b) met-rbc (red) in flow. the thicker line shows the mean spectra of the normal and met rbcs and the shaded region show the standard deviation in the recorded spectra. the vertical line shows the band at 1358 cm-1.