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Caption : figure s1 – characterization of ifn-γ-nps by dls. a. ifn-γ-nps were prepared at different molar ratios of ifn-γ (0.1, 0.25, 0.35, 0.5 and 0.7), maintaining ch and γ-pga molar ratios constant, 1 and 1.5, respectively. after preparation, their size (d, nm), polydispersity index (pdi) and ζ potential (ζ pot., mv) measurements were determined by dynamic light scattering (dls). b. particle size distribution plots from a monodisperse (1:0.25:1.5) and a multidisperse preparation (1:0.7:1.5) were obtained by intensity of the scattered light, using the same equipment. 

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Caption : figure s2 – ifn-γ-nps are internalized by macrophages. human macrophages were stimulated with il-10 alone or in combination with ifn-γ-nps for 72 h. cells were recovered and fixed for imaging flow cytometry. a. the percentage of nps internalization was determined after application of an internalization mask on the cell population of positive cells for fitc(ft) ft(ifn-γ-nps). each cell is represented by a row of three images acquired simultaneously in flow, from left to right: brightfield with a cytoplasm mask represented in blue, fitc fluorescence (green) for the ft(ifn-γ-nps), merged image (scale bars, 10 µm). b. representative dot plot and histogram profile for the ft(ifn-γ-nps) internalization by macrophages (lower panel) by imaging flow cytometry. data is representative of 2 donors. 

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Caption : figure s3 - mixed lymphocyte reaction controls. lymphocytes isolated from a blood donor different from those used for monocyte isolation were labeled with allogeneic cfse and cultured alone (-) or with dcs differentiating cytokines (gm-csf and il-4), lps, il-10, ifn-γ, nps, ifn-γ-nps or pha as experimental controls. after 7 days of co-culture, cells were surface stained for cd3, cd4 and cd8 followed by flow cytometry analysis. a. the percentage of cd4+ and b. cd8+ dividing cells were determined. comparisons were performed using the kruskal-wallis test followed by dunn’s multiple comparisons test (**p<0.01; ***p<0.001; ****p<0.0001).