File Name : slide5.jpg

Caption : figure s1) coordinated activity failed to develop in spheroids composed of 2,000 cells; emerged by day 21 in 4k spheroids. a) activity was not detected in 2k spheroids. representative δf/f traces from 2,000 cell spheroids at day 7 (top), day 14 (center) and day 21 (bottom). time series scale bars are 5 sec (horizontal) by 0.1 δf/f (vertical).  b) some sporadic activity was evident in 4k spheroids. representative δf/f traces from 4k spheroids at day 7 (top), day 14 (center) and day 21 (bottom). time series scale bars are 5 sec (horizontal) by 0.1 δf/f (vertical).  c) percent of cells active, correlation of active cells, amplitude of events, and frequency of events were calculated for 4k spheroids imaged at day 21 (n = 18). marker symbol indicates the sex of rodent the spheroid was derived from (▲=♂, ●=♀). marker color ranges from violet to maroon and indicates which data points came from the same dissections. t-tests without assuming equal variance were conducted and found no significant differences between means of male- and female-derived spheroids in any category with α=0.01. red + symbols indicate statistically detected outliers.