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Caption : figure s1 establishment of lps-induced intestinal epithelial cells injury model. (a) different action time of lps (n = 8). (b) different concentrations of lps (n = 8). (c) the morphology of ipec-j2 cells (n = 6). (d-f) the il-1β level in ipec-j2 cells exposed to lps for 24 h, 48 h and 72 h (n = 6). (g-h) the il-1β level in ipec-j2 cells exposed to lps after administration of senps for 24 h and 48 h (n = 6). all data are expressed as mean ± s.e.m., *p< 0.05, **p<0.01, ***p<0.001.

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Caption : figure s2 effects of senps on the cytotoxity in ipec-j2 cells induced by lps. (a) cell apoptosis detected by annexin v-fitc and pi staining. (b) quantitative statistical analysis results. all data are expressed as mean ± s.e.m., n=6, *p< 0.05, **p<0.01, ***p<0.001.

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Caption : figure s3 the expression level of tbc1d15 in ipec-j2 cells transfected with tbc1d15 sirna. (a-b) screening of rna sequences and quantitative analysis results (n = 3). (c-d) screening of rna concentration and quantitative analysis results (n = 3). (e-f) the expression levels of upstream and downstream genes in ipec-j2 cells transfected with tbc1d15 sirna and quantitative analysis results (n = 3). (g) the morphology of ipec-j2 cells transfected with tbc1d15 sirna (n = 6). (h) the cell viability of ipec-j2 cells transfected with tbc1d15 sirna (n = 8). all data are expressed as mean ± s.e.m., *p< 0.05, **p<0.01, ***p<0.001.