File Name : tab s1.tiff

Caption : tableau s1: summary table of the various synthesis batches carried out for the in vivo degradation study.

File Name : fig s1.tiff

Caption : figure s1: follow-up of the maximal light intensity on in vivo acquisition, corresponding to the first ten seconds of recorded luminescence decays. the ground control (black dashed line) corresponds to the mean maximal intensity obtained with the control group. error bars represent the standard deviation (n= 6).

File Name : fig s2.tiff

Caption : figure s2: quantification of the maximum intensity of the control group luminescence on ex vivo. the presented values correspond to the first ten seconds of the luminescence decays recorded, for each organ. statistical differences were assessed by using multiple t-tests analysis. the error bars represent the standard deviation (n = 6).

File Name : fig s3.tiff

Caption : figure s3: zgooh (a) and zgopeg (b) icp-ms quantification in each organ over the time. the percentage of injected dose has been calculated for the zinc content quantification regarding theoretical original injected amount. control group quantification in each organ over the time calculated from the ga (c) or zn content (d). the error bars represent the standard deviation (n = 6). ratios of ga/zn quantified contents from zgooh (e) and zgopeg (f) groups have been plotted to assess and validate the results. a theoretical ratio of 2 should be obtained. statistical differences between anterior adjacent time-points within each group were assessed by using multiple t-tests analysis. discovery determined using the two-stage linear step-up procedure of benjamini, krieger and yekutieli, with q = 1%. each row was analyzed individually, without assuming a consistent sd (****: p <0.0001; ***: p <0.001; **: p <0.01; *: p <0.1; ns: p> 0.1). number of t tests: 6.

File Name : fig s4.tiff

Caption : figure s4: to assess the correlation between the normalized luminescence and the ga and zn content, normal gaussian distribution was first tested. to do so, anderson darling test, d’agostino pearson omnibus normality test, shapiro-wilk normality test and kolmogorov-smirnov normality test with dallas-wilkinson-lilliefor p value were used. due to the absence of a normal distribution, non-parametric spearman test was used. (a) presents an example of the obtained results for the liver of zgooh group. (b) presents a summary of the obtained results for the spearman correlation tests (****: gp <0.0001; ***: gp: 0.0002; **: gp: 0.0021; *: gp: 0.0332; ns: gp: 0.1234).

File Name : fig s5.tiff

Caption : figure s5: monitoring and quantification of the area of zgooh and zgopeg aggregates observed in liver tissue. the mustache boxes represent the populations between the percentiles 10 and 90%; the middle line represents the mean. values outside the 10-90 percentiles are represented by dots. hotspots were imaged and approximately 10 images per time per group were analyzed, resulting in the detection of approximately 30 aggregates per group per time. the significance of the results between adjacent time-points within each group was carried out using the mann-whitney statistical test compared to time h1 (****: p <0.0001; ***: p <0.001; **: p <0.01; *: p <0.1; ns: p> 0.1). the error bars represent the standard deviation.

File Name : fig s6.tiff

Caption : figure s6: zgooh (top) and zgopeg (bottom) size distribution over the time extracted from tem imaging (n>100). normal gaussian distribution was first tested. to do so, anderson darling test, d’agostino pearson omnibus normality test, shapiro-wilk normality test and kolmogorov-smirnov normality test with dallas-wilkinson-lilliefor p value were used. due to the absence of a normal distribution, non-parametric kolmogorov-smirnov test comparing cumulative distribution was used between anterior adjacent time-points within each group (****: gp <0.0001; ***: gp: 0.0002; **: gp: 0.0021; *: gp: 0.0332; ns: gp: 0.1234).

File Name : fig s7.tiff

Caption : figure s7: monitoring of the body mass of the mice following the injection of zgooh (blue line), zgopeg (red line) or 5% glucose (control group, black line) over time. the error bars represent the standard deviation (n = 6).

File Name : fig s8.tiff

Caption : figure s8: monitoring of different biochemical markers and blood analysis of mice after injection of zgooh (blue), zgopeg (red) and the control group (black). the concentrations of direct bilirubin (a), red blood cells (b) and hematocrit (c) were examined. the rate of white blood cells (h), the platelet concentration (i), the percentage of lymphocytes (j) and the percentage of monocytes (k) and the average globular volume (l) were examined. statistical differences with the control group at each time-point were assessed by using two-way anova analysis. (****: p <0.0001; ***: p <0.001; **: p <0.01; *: p <0.1; ns: p> 0.1). the error bars represent the standard deviation (n = 6).