File Name : figure s1 topography.tiff

Caption : fig. s1 determination of the polyscopoletin nanofilm thickness by local removal of the polymer from the gold surface in a rectangular area (left) to reveal the cross-section (right). 

File Name : figure s1 cross section.tiff

Caption : fig. s1 determination of the polyscopoletin nanofilm thickness by local removal of the polymer from the gold surface in a rectangular area (left) to reveal the cross-section (right). 

File Name : figure s2 low current sensitivity mode.tiff

Caption : fig. s2 current maps of the polyscopoletin film (nip) by conducting afm in low (left) and high (right) current sensitivity mode that shows the excellent electrical insulating property of the film.

File Name : figure s2 high current sensitivity mode.tiff

Caption : fig. s2 current maps of the polyscopoletin film (nip) by conducting afm in low (left) and high (right) current sensitivity mode that shows the excellent electrical insulating property of the film.

File Name : figure s3.tiff

Caption : fig. s3 high resolution topographic and current maps of g-peptide (5 µm) imprinted polyscopoletin nanofilms after template removal recorded by conducting afm.

File Name : fig s4.tif

Caption : fig. s4 ir absorbance spectra of individual steps of the mip formation on seira gold surface. trace 1 shows the adsorption of g-peptide highlighted by the corresponding amide i and amide ii bands at 1673 cm-1 and 1517 cm-1 (blue rectangle), respectively. the observed bands in trace 2 are attributed to the carbonyl ν(c=o) stretching vibration (1715 cm–1), aromatic and vinyl ν(c=c) stretching modes (1600 – 1400 cm−1), ν(c–o) stretching vibration of the ester group (1280 cm−1), the ν(c–o–c) stretching of alkyl ethers (1160 cm−1) and δ(c–h) bending modes (1400 – 1000 cm−1), all of these are characteristic of the polyscopoletin film. electrochemical template removal (trace 3) is monitored through the appearance of a broad absorption band at ca. 1640 cm–1 attributed to the δ(o–h) bending vibrations (red rectangle) of water molecules reaching the gold surface via the created mip cavities (trace 4). subsequent rebinding of the egfn shows only the appearance of water molecules reaching the au surface as highlighted by the δ(o–h) bending vibration mode.

File Name : fig s5a.tif

Caption : fig. s5 comparison of binding of rbd (a) and spike protein (b) to the mip measured in 5 mm k3[fe(cn)6]/k4[fe(cn)6] in pbs or 1:20 diluted hotgen buffer.

File Name : fig s5b.tif

Caption : fig. s5 comparison of binding of rbd (a) and spike protein (b) to the mip measured in 5 mm k3[fe(cn)6]/k4[fe(cn)6] in pbs or 1:20 diluted hotgen buffer.

File Name : fig s6.tif

Caption : fig. s6 ir absorbance spectra of individual steps of the nip formation on seira gold surface. the observed bands in trace 1 are attributed to the carbonyl ν(c=o) stretching vibration (1715 cm–1), aromatic and vinyl ν(c=c) stretching modes (1600 – 1400 cm−1), ν(c–o) stretching vibration of the ester group (1280 cm−1), the ν(c–o–c) stretching of alkyl ethers (1160 cm−1) and δ(c–h) bending modes (1400 – 1000 cm−1), all of these are characteristic of the polyscopoletin film. subsequent binding of the g-peptide leads to the appearance of amide i and ii, detectable from the corresponding difference spectrum (trace 3) between trace 2 and trace 1.