File Name : figure s1.tif

Caption : bar diagram to compare the intensity of the fluorescence signal produced by different rna g-quadruplexes. error bars represent the standard deviation of three different samples.

File Name : figure s2.tif

Caption : effect of different kcl concentrations on the intensity of the fluorescence emission of lutd.

File Name : figure s3.tif

Caption : bar diagram to visualize the effect of the incubation time on the fluorescence signal to determine the shortest incubation time required to achieve the highest intensity fluorescence signal. error bars represent the standard deviation of three different samples.

File Name : figure s4.tif

Caption : a., b. & c. plot representing the deviation of the signal intensity of 3 different mirnas in 10% human serum for diluting concentrations. the error bar represents the standard deviation of three different spiked samples.

File Name : figure s5.tif

Caption : bar diagram for analysis of the mlp probe concentration required for the system. error bars represent the standard deviation of three different samples.

File Name : figure s6.tif

Caption : 16% denaturing page for optimization of the ligation analyzed by varying the splintr ligase concentrations. lane 1: mlp probes + target (1 + 2 + 3) + splintrl 0 unit; lane 2: : mlp probes + target (1 + 2 + 3) + splintrl 6.25 unit; lane 3: mlp probes + target (1 + 2 + 3) + splintrl 12.5 unit; lane 4: : mlp probes + target (1 + 2 + 3) + splintrl 25 unit; lane 5: : mlp probes + target (1 + 2 + 3) + splintrl 50 unit. 

File Name : figure s7.tif

Caption : 16% denaturing page analyzing the effect of target sequence changes on ligation efficiency. lane 1: mlp probes + target (1 + 2 + 3); lane 2: mlp probes + target (1_sequence changed + 2 + 3) + splintrl; lane 3: mlp probes + target (1 + 2_sequence changed + 3) + splintrl; lane 4: mlp probes + target (1 + 2 + 3_sequence changed) + splintrl; lane 5: mlp probes + target (1 + 2 + 3) + splintrl.

File Name : figure s8.tif

Caption : a., b. & c. plot presenting the deviation of the signal intensity for each mirna target when other targets are present in 10 pm concentrations. the limit of detection (lod) for target 1 (mir21), target 2 (146a), and target 3 (25b) was found respectively 1.38 fm, 1.93 fm, and 2.04 fm. the error bar represents the standard deviation of three different samples.

File Name : figure s9.tif

Caption : a., b. & c. plot presenting the deviation of the signal intensity for each mirna target when other targets are present in 1 pm concentrations. the limit of detection (lod) for target 1 (mir21), target 2 (146a), and target 3 (25b) was found respectively 6.55 fm, 11.86 fm, and 21.41 fm. the error bar represents the standard deviation of three different samples.

File Name : figure s10.tif

Caption : bar diagram representing the limit of detection when all three mirna targets are equally diluted at the same time. the data shows that for dilution of each mirna until 100 fm concentration, the signal could be differentiated compared to samples with no target. the error bar represents the standard deviation of three different samples.