File Name : fig.s1.tif
Caption : fig.s1. native page image of the products of the amplification reactions with the untreated padlock probes and those treated with t4 dna ligase. m represented dna marker (25-500 bp). lanes 1 and 2: amplicons of the reaction with untreated padlock probes for s. aureus 16s rdna and sars-cov-2 n gene detection; lanes 3 and 4: amplicons of the reaction with the corresponding padlock probes treated with t4 dna ligase.
File Name : fig.s2.tif
Caption : fig.s2. validation of the rc-sea principle. native page analysis image of rc-sea process for (a) s. aureus 16s rdna and (b) sars-cov-2 n gene detection. lane 1: target + primers + dps; lane 2: target + primers; lane 3: target + dps; lane 4: target; lane 5: primers + dps; lane 6: primers; lane 7: dps. m represented dna marker (25-500 bp).
File Name : fig.s3.tif
Caption : fig.s3. changes of fluorescence curves of rc-sea with (a) different amounts of sea primers, (b) different primer ratios, (c) dps of different lengths, (d) different amounts of dps, (e) different dosages of bst dna polymerase, (f) different amount of dntps, as well as (g) those performed at different temperature. ntc represented the no template control. all parameters remained the same except that which was to be optimized. error bars represent the standard deviations of three independent measurements.
File Name : fig.s4.tif
Caption : fig.s4. fluorescence curves of (a) sea and (b) lamp for s. aureus 16s rdna detection in dna extracts from 10-fold serial dilutions of the s. aureus suspension from 108 to 102 cfu/ml. fluorescence curves of (c) sea and (d) lamp for sars-cov-2 n gene detection in 10-fold serial dilutions of sars-cov-2 rna from 108 to 102 copies/ml. ntc represented no template control. error bars represent the standard deviations of three independent measurements.
File Name : fig.s5.tif
Caption : fig.s5. sensitivity of rc-sea based colorimetric assays to dna and rna targets. rc-sea, sea, and lamp based colorimetric assays for (a) s. aureus 16s rdna detection in dna extracts of 10-fold serial dilutions of the s. aureus suspension from 108 to 103 cfu/ml, and (b) sars-cov-2 n gene detection in 10-fold serial dilutions of sars-cov-2 rna from 108 to 103 copies/ml. ntc represented no template control.