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Caption : figure s1. schematic diagram and photos of the device. (a) schematic diagram of the device. (b) photos of the device showing its basic construction, including top view (left) and side view (right).

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Caption : figure s2. acquisition of metabolite profiles from single cells. (a) eic of pc (34:1) of pbmcs in the positive ion mode. (b) heatmap of the ions detected in 679 pbmcs. (c) umap analysis of hela cells, hek293 cells and mixed cells. (d) heatmap of the ions detected in mixed cancer cells of hek293 cells and hela cells.

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Caption : figure s3. proof of stability. (a) umap analysis of t cells from different batches. (b) umap analysis of nk cells from different batches.

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Caption : figure s4. classification of detected metabolites in lymphocytes in the positive mode.

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Caption : figure s5. distribution of acetylcarnitine (left) and sm(d44:2) (right) in t cells, b cells and nk cells.

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Caption : figure s6. the metabolic pathway difference between t cells and b cells, including (1) sphingophospholipid metabolism pathway, (2) glutathione metabolism pathway and (3) histidine metabolism pathway.

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Caption : figure s7. logarithmic radar chart of 40 differential metabolites in t cells, b cells, nk cells and monocytes.

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Caption : figure s8. schematic diagram of the procedure of preparing mixed lymphocytes.

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Caption : figure s9. proof of accuracy and repeatability. (a, b) umap analysis of mixed cells on the same day. (c) umap analysis of pbmc on the same day. (d) umap analysis of mixed cells on different days.