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Caption : fig. s1. esi-ms spectra of aptamer mnk2f modifications. a. mnk2f azide; b. mnk2f amino; c. mnk2f thiol.

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Caption : fig. s2. uv-vis spectrum characterization of peg-dbco reagent and aptamer conjugate dbco-n3. characteristic bands at 292 and 310 nm of dbco decay once it is conjugated to azide. a shoulder band at 312 nm in the aptamer conjugated could be attributed to the formation of the triazole linkage.

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Caption : fig. s3. uv-vis spectra of dspe-peg-maleimide and its corresponding aptamer conjugate maleimide-sh.

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Caption : fig. s4. agarose gel electrophoresis to illustrate covalent conjugation between functionalized-peg and modified-aptamer. the absence of the characteristic aptamer band in the well named as “ne:2f-nh2 after dialysis” confirms the formation and isolation of the nanoconjugate.

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Caption : fig. s5. agarose gel electrophoresis to follow carbodiimide reaction and assess edc effect on the resulting conjugate. 1. apmnk2f-nh2 – characteristic band of the free aptamer; 2. peg-cooh + apmnk2f-nh2 – characteristic band of the lipid-aptamer conjugate; 3. ne – characteristic band (absence) of the blank nanoemulsion (ve:sm:peg); 4. 5. and 6. ne:apmnk2f-nh2 5:1 – characteristic bands of the aptamer-conjugated nanoemulsion when: edc is not removed (4. +edc); after amicon ultrafiltration to remove edc (5. -edc); after dialysis purification (6. dialysis). strong intensity of the band in well 4 compared to weak band in well 5 reveals the reversibility effect of the edc when it is not removed from the reaction medium. additionally, only a weak shadow is noticed after purification by dialysis is performed, proving that the aptamer is all conjugated to the nanoemulsion and no free aptamer is detected.

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Caption : fig. s6. flow cytometry analysis showing percentage of cells internalizing top fluor®-sm nanosystems over time (30 minutes, 1 hour and 4 hours).

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Caption : fig. s7. quantification of annexin v assay for apoptosis detection by measuring mean fluorescence intensity using fiji image j software. data were represented in terms of %mfi relative to positive control (control+).