File Name : ___32.tif

Caption : supplementary figure s1. prokaryotic expression, protein purification and preparation of sopb and sope2 antibodies. a. a model of the target sequence inserted into the prokaryotic expression vector pet-28a(+). b. the process used to prepare the antibodies. c. salmonella genomic dna. lanes 1, 2, and 3 represent three replicates; lane 4 is a negative control. d. diagram of sopb and sope2 gene amplification. lanes 1, 2, and 3 represent the sopb gene at approximately 1,686 bp and the sope2 gene at approximately 723 bp, respectively. e. sds‒page diagram. f. recombinant proteins obtained after elution with 80 mm imidazole. g. antibody titer. h. verification of the purified sopb and sope2 antibodies.