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Caption : supplementary material figure 1. detection of yeast cells. detection and counting of yeast cells was done using the morpholibj plugin function gray scale attribute filtering with top hat in fiji v1.53t. here we show an example of an image in bf microscopy and the corresponding image after thresholding. the red dots on black background show the yeast cells detected and counted. white scale bar is at 100 µm.

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Caption : supplementary material figure 2. cytometry histograms to estimate apparent affinity. the apparent affinity of the nb on the yeast surface was estimated. nef19+ & cd16.21+ were incubated with either their cognate or irrelevant antigen and differing concentrations. each concentrations used are indicated on the corresponding row. values used in main text fig 1c were taken here gated using the lowest concentration on the irrelevant antigen.

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Caption : supplementary material figure 3. yeast driven along the channel surface. a schematic showing the expected interaction between the flowing yeast cell on the channel surface and the antigen functionalized on the channel surface (image not to scale).

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Caption : supplementary material figure 4. bright field (bf), fluorescence (fluo) and capture efficiency y. (a) the fraction of cell count postflow to preflow of the pure non-binding yeast (negative) used as control during enrichment experiments imaged through bf microscopy. (b) the fraction of cell count postflow to preflow of the pure binding yeast (positive) used as control during enrichment experiments imaged through bf microscopy. (c) the calculated capture efficiency y using the bf data using eq 2 of the main text. (d) the fraction of cell count postflow to preflow of the pure non-binding & fluorescent yeasts (negative) in the mixture during enrichment experiments imaged through fluo microscopy. (e) the fraction of cell count postflow to preflow of the pure non-binding & non-fluorescent yeast (positive) in the mixture during enrichment experiments imaged through fluo microscopy. (f) the calculated capture efficiency y using the fluo data using eq 2 of the main text.