File Name : fig. s1.tif

Caption : fig. s1 (a) 3d fem analysis of a hypersonic resonator at 1.58 ghz in the chamber and velocity field (b) simulation on x-z plane. color bar indicates the distribution of fluid velocity, and red arrows describe flow direction (c) under the as with the chamber height of 2000 μm, the confocal image of the cell under the stimulation of as. green indicates the cell membrane. blue circle and dotted line indicate the range of action and its diameter. scale bar = 300 μm.

File Name : fig. s2.tif

Caption : fig. s2 (a) 2d fem analysis of 1.58 ghz hypersonic resonator with different input power at the chamber height of 500 μm. (b) data diagram of velocity and height of acoustic streaming.

File Name : fig. s3.tif

Caption : fig. s3 time course of as inducing cells to move with blebs (between 180 and 240 seconds). the yellow arrow and yellow boxes indicate the blebs. scale bar= 10 μm.

File Name : fig. s4.tif

Caption : fig. s4 the changes of ne-4c stem cells with time under ultra high as stimulation. the data are expressed as the mean ± sd (n = 3). scale bar = 25 μm.

File Name : fig. s5.tif

Caption : fig. s5 schematic diagram of as affects the cellular cytoskeleton.

File Name : fig. s6.tif

Caption : fig. s6. the images of cells which untreated and stimulated by different intensities of as for 8 min. scale bar = 75 μm.

File Name : fig. s7.tif

Caption : fig. s7. (a) the image and data statistical chart (b) of proliferation cells which untreated and stimulated by medium as once for different stimulation time. the data are expressed as mean ± sd (n = 3). 

File Name : fig. s8.tif

Caption : fig. s8. corresponding system conditions for regulating cell behavior.