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Caption : supplementary figure s1 transmission electron microscopy of thp-1 cells treated with (a) sync or (b) untreated controls (a) tem images of thp-1 cells preserved with hpf/fs after incubation with cfe = 6.5 mm sync for 10 min. iron oxide sync particles are visible as electron-dense structures. (b) thp-1 cells after hpf/fs without the addition of sync. no electron-dense structures can be identified in control cells.  

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Caption : supplementary figure s2 magnetic particle spectroscopy of sync with (a) chondroitin sulfate, (b) heparin, (c) hyaluronic acid with low molecular weight, (d) hyaluronic acid with high molecular weight. baseline a5/a3 ratio of sync in dh2o is shown in grey. mps measurements were performed at bexit = 12 mt and boff = 0.05 mt for 667 s at 37°c. (a) chondroitin sulfate caused an a5/a3 increase by ∆0.02% ± 0.11% after 667 s. (b) the presence of heparin decreased the a5/a3 ratio by ∆-0.11% ± 0.07% during the measurement. (c) low molecular weight hyaluronic acid increased the ratio by ∆0.11% ± 0.10% compared to sync in dh2o. (d) whereas high molecular weight hyaluronic acid decreased the ratio by ∆-0.09% ± 0.34% after 667 s. 

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Caption : supplementary figure s3 differential glycosaminoglycan staining using stains-all. 1) lb = loading buffer 2) cs = 5 µg chondroitin sulfate; 3) hs = 5 µg heparin; 4) ha = 10 µg high molecular weight hyaluronic acid; 5) empty 6) glycosaminoglycans isolated from 15×106 thp-1 cells; 7) glycosaminoglycans isolated from 15×106 cho-k1 cells; 8) glycosaminoglycans isolated from 15×106 psga-745 cells; 9) lb = loading buffer.

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Caption : supplementary figure s4 differential glycosaminoglycan staining after gag digestion using stains-all. 1) lb = loading buffer 2) cs = 5 µg chondroitin sulfate; 3) hs = 5 µg heparin; 4) ha = 10 µg high molecular weight hyaluronic acid; 5) glycosaminoglycans isolated from 4×106 thp-1 cells; 6) glycosaminoglycans isolated from 4×106 thp-1 cells pretreated with 10 mu chondroitinase abc for 1h at 37°c 7) glycosaminoglycans isolated from 4×106 thp-1 cells.