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Caption : a comparison of the saxs peaks expressed for the lys/tre/h2o and lys/suc/h2o systems at different ph and incubation temperatures

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Caption : waxs data of tre/h2o and suc/h2o solutions compared to pure milli-q-water. the sugar:water ratio was same in these systems as in the three-component systems.

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Caption : afm image of lys/h2o at ph 3.5 including colour map.

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Caption : afm image of lys/tre/h2o at ph 3.5 including colour map.

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Caption : afm image of lys/suc/h2o at ph 3.5 including colour map.

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Caption : afm image of lys/h2o at ph 2.0.

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Caption : afm image of lys/h2o at ph 2.0 including colour map.

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Caption : afm image of lys/tre/h2o at ph 2.0 including colour map.

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Caption : afm image of lys/suc/h2o at ph 2.0 including colour map.

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Caption : representative afm images of (a) lys/suc/h2o and (b) lys/tre/h2oat ph 2.0 , and (c) lys/suc/h2o and (d) lys/tre/h2o at ph 3.5. the sucrose containing systems have been incubated at 68 ◦c and the trehalose systems at 65◦c.

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Caption : denaturation temperatures for the lys/h2o, lys/tre/h2o, and lys/suc/h2o systems without any prior incubation or altering of ph as
a function of the protein concentration. the sugar:water weight ratio was kept constant in all three-component systems. the measurements were performed at a heating rate of 2 ◦c/min.

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Caption : representative dsc curves for the lys/h2o, lys/tre/h2o, and lys/suc/h2o systems at ph 2.0 (a) and ph 3.5 (b) of the crystallisation event measured at a cooling rate of 10 ◦c/min. the curves have been vertically shifted for clarity.

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Caption : representative dsc curves for the unfolding (a) and folding (b) of the lys/h2o systems at ph 2.0.

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Caption : representative dsc curves for the unfolding (a) and folding (b) of the lys/tre/h2o systems at ph 2.0.

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Caption : representative dsc curves for the unfolding (a) and folding (b) of the lys/suc/h2o systems at ph 2.0.

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Caption : representative dsc curves for the unfolding (a) and folding (b) of the lys/h2o systems at ph 3.5.

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Caption : representative dsc curves for the unfolding (a) and folding (b) of the lys/tre/h2o systems at ph 3.5.

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Caption : representative dsc curves for the unfolding (a) and folding (b) of the lys/suc/h2o systems at ph 3.5.