LOCUS plasmid 6057 bp DNA circular SYN 15-OCT-2024 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE . ORGANISM . . COMMENT Annotated with pLannotate v1.2.2 FEATURES Location/Qualifiers polyA_signal complement(1446..2068) /note="pLannotate" /label="hGH poly(A) signal" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="polyA_signal" rep_origin complement(4411..4839) /note="pLannotate" /label="f1 ori" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="rep_origin" promoter 4972..5329 /note="pLannotate" /label="SV40 promoter" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="promoter" CDS complement(2112..3380) /note="pLannotate" /label="OPN5-Snorkel" /database="swissprot" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" promoter complement(3486..3685) /note="pLannotate" /label="CMV promoter" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="promoter" promoter 4866..4970 /note="pLannotate" /label="AmpR promoter" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="promoter" CDS join(5364..6057,1..101) /note="pLannotate" /label="NeoR/KanR" /database="snapgene" /identity="99.9" /match_length="100.0" /fragment="False" /other="CDS" rep_origin 709..1297 /note="pLannotate" /label="ori" /database="snapgene" /identity="99.8" /match_length="100.0" /fragment="False" /other="rep_origin" enhancer complement(3687..4066) /note="pLannotate" /label="CMV enhancer" /database="snapgene" /identity="99.5" /match_length="100.0" /fragment="False" /other="enhancer" promoter complement(3439..3457) /note="pLannotate" /label="T7 promoter" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="promoter" CDS 4226..4380 /note="pLannotate" /label="lacZalpha (fragment)" /database="snapgene" /identity="100.0" /match_length="89.1" /fragment="True" /other="CDS" polyA_signal 333..380 /note="pLannotate" /label="HSV TK poly(A) signal" /database="snapgene" /identity="98.0" /match_length="100.0" /fragment="False" /other="polyA_signal" CDS 122..274 /note="pLannotate" /label="ble (fragment)" /database="swissprot" /identity="100.0" /match_length="40.5" /fragment="True" /other="CDS" polyA_signal complement(471..487) /note="pLannotate" /label="bGH poly(A) signal (fragment)" /database="snapgene" /identity="100.0" /match_length="8.2" /fragment="True" /other="polyA_signal" ORIGIN 1 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 61 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 121 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 181 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 241 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa 301 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca 361 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga 421 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca 481 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc 541 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa 601 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 661 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 721 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 781 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 841 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 901 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 961 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 1021 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 1081 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 1141 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 1201 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 1261 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 1321 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 1381 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gctggccgcg 1441 tacccaattc aacaggcatc tactgagtgg acccaacgca tgagaggaca gtgccaagca 1501 agcaactcaa atgtcccacc ggttgggcat ggccaggtag cctatgctgt gtctggacgt 1561 cctcctgctg gtatagttat tttaaaatca gaaggacagg gaagggagca gtggttcacg 1621 cctgtaatcc cagcaatttg ggaggccaag gtgggtagat cacctgagat taggagttgg 1681 agaccagcct ggccaatatg gtgaaacccc gtctctacca aaaaaacaaa aattagctga 1741 gcctggtcat gcatgcctgg aatcccaaca actcgggagg ctgaggcagg agaatcgctt 1801 gaacccagga ggcggagatt gcagtgagcc aagattgtgc cactgcactc cagcttggtt 1861 cccaatagac cccgcaggcc ctacaggttg tcttcccaac ttgccccttg ctccatacca 1921 cccccctcca ccccataata ttatagaagg acacctagtc agacaaaatg atgcaactta 1981 attttattag gacaaggctg gtgggcactg gagtggcaac ttccagggcc aggagaggca 2041 ctggggaggg gtcacaggga tgccacccgg gatctgttca ggaaacagct atgaccgcgg 2101 ccggccgttt aggcataatc aggcacatca taggggtatt gcatcccttt gtcgtcatcg 2161 tccttgtagt cgcttcccca aagcatgatc aggatgatca ggctaatgac ggtgagtacg 2221 accaatgcca gtatggcgga aatcaccaca actttccacc taatctcgta tcttggcttc 2281 ttctgggatc cactgccaga agaaccgctt gaagcgcctt cccactcttc atgaatttcc 2341 agcacagcag aagacttcct gactgtggtt acggtgtgca gcctgaagcc ttccagagac 2401 ttcttcttgg ttgctttcaa accaccagtt tggcaacagg caaatttgta atcaataact 2461 tggtaaatga tgggattgta catcgctgca gattttgcaa gtagggttgg caccacagag 2521 agctgtatgg gaatggagtc tggccttcca aaagctgacc acacagacac cactgcataa 2581 ggaatccagg caatcaggaa tccagcacaa atcaacatcg ctacctttgt cagtttcatt 2641 tccagcacat ggctgctatg gatccgactg tcgaaatgag ctacttcttt ggaagaggac 2701 ttaaccttgg caatgatctt tacgtaggag aacacgatca cagccgttgg gagcaagagg 2761 cagaagaaga ggatgttcag gatgaaaacc tggcccccta ccgaggcctg ggccagccac 2821 cagtccaggg tgcacgaggt tccgaagggc tcaggtacgt agtcccccag acctaccaag 2881 ggcatggtgg tccagaagga agcataggcc cagatggctg ccaggcagat gtaggcgtgc 2941 tttcttttca gccaaacccc ataagataaa tagcagattt tcaaatatcg atccaggctg 3001 acagcagtca tggtgataag gcttccacag ccaaagaaaa atccagccca tccataccag 3061 cggcagccga tccagccaaa cacccagcgg tgacaaaagc aagagatgat ggtgaacggc 3121 ttgcctacaa ctgaaatccc cagatcacag actgctaaat tgatagtcat tatttcagcg 3181 ggtctcagct tcttctttcg tctagaagac atgtaaagga catatccatt tccaaatgtg 3241 gacagaatcc caattattgt taggtaaaag ccagccacta aatccgcttc ccaagaaagt 3301 ttggaagcaa aaggatcccc atctcgaagg taatggggca ggcgctcgtc ctgaggcagg 3361 gcagtgtgat ttaacgccat ggcgatcgcg gcggcagatc tcctcggtac cggatccagt 3421 cgacgaattc ccggccgccc tatagtgagt cgtattacaa aattctgacg gttcactaaa 3481 cgagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaacggg 3541 gcggggttat tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca 3601 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca 3661 ttggtgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac 3721 tgccaagtag gaaagtcccg taaggtcatg tactgggcat aatgccaggc gggccattta 3781 ccgtcattga cgtcaatagg gggcggactt ggcatatgat acacttgatg tactgccaag 3841 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt 3901 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca 3961 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat 4021 gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcaaca tggcggtcat 4081 attggacatg agccaatata aatgtacata ttatgatata gatacaacgt atgcaatggc 4141 caatagccaa tattgattta tgctatataa ccaatgacta atatggctaa ttgccaatat 4201 tgattcaatg tatagatcag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa 4261 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 4321 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 4381 tggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 4441 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 4501 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 4561 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 4621 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 4681 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 4741 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 4801 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa 4861 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 4921 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg 4981 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 5041 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 5101 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 5161 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 5221 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 5281 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag 5341 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 5401 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 5461 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 5521 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 5581 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 5641 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 5701 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 5761 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 5821 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 5881 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 5941 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 6001 gtgtggcgga tcgctatcag gacatagcgt tggctacccg tgatattgct gaagagc //