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Caption : figure s1 uptake and cytotoxicity assay of gppf. (a)dil-stained gppf were uptake by thp-1 macrophage after 4h incubation. scale bars,100μm. (b) cck8 assay kit showed gppf exerted mere toxic effect on cells across various concentrations and incubation times. (c) the conjugation of gppf with the two plasmids at various n/p ratios (2, 5, and 10) was evaluated using gel electrophoresis analysis.

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Caption : figure s2. reactome pathway analysis of differentially expressed genes (degs) in m2 subtypes compared to the control(m0). reactome pathway differences of (a)up-regulated degs or (b)down-regulated degs in m2(gppf), m2a(gppf/pil4), and m2c(gppf/pil10) macrophages. visualization of ppi networks and representative reactome pathway of degs in (c)m2a(gppf/pil4) and (d)m2c(gppf/pil-10) using cytoscape software. enrichment padj<0.05. 

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Caption : figure s3. identification and functional analysis of up-regulated genes in m2a(gppf/pil4) and m2c(gppf/pil10) compared to m0 macrophage. (a)up-regulated differentially expressed genes (degs) were analyzed for co-expression and unique expression using a venn diagram. (b) biological process uniquely enriched in m2a(gppf/pil4) macrophages. (c) biological process uniquely enriched in m2c(gppf/pil10) macrophages. (d)biological process revealed that up-regulated co-expressed degs in m2a(gppf/pil4) and m2c(gppf/pil10) were involved in positive regulation of type 2 immune response, antimicrobial and antiviral process. (e)wikipathway revealed that up-regulated co-expressed degs in m2a(gppf/pil4) and m2c(gppf/pil10) were involved in anti-inflammatory effects on sars-cov-2. enrichment padj<0.05. 

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Caption : figure s4. identification and functional analysis of down-regulated genes in m2a(gppf/pil4) and m2c(gppf/pil10) compared to m0 macrophage. (a)down-regulated differentially expressed genes (degs) were analyzed for co-expression and unique expression using a venn diagram. (b)biological process revealed that down-regulated co-expressed degs in m2a(gppf/pil4) and m2c(gppf/pil10) were involved in positive regulation of cell cycle and negative regulation of bmp signaling. (c)wikipathway revealed that down-regulated co-expressed degs in m2a(gppf/pil4) and m2c(gppf/pil10) were involved in cell cycle and cancer network. enrichment padj<0.05.