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Caption : figure. s1  fluorescence characterization of dual antibody-modified magnetic beads. (a) magnetic beads modified with fitc-labeled cd9 antibody, (b) magnetic beads modified with pe-labeled cd63 antibody, and (c) fluorescence image of merged.

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Caption : figure. s2  characterization of evs. (a) tem image of a549-derived evs. (b) tem image of hl60-derived evs. (c) nta analysis of the size distribution of evs derived from a549 cells. (d) nta analysis of the size distribution of evs derived from hl60 cells. (e) western blotting displays the expression of protein markers (cd63, cd9, tsg101, and calnexin) in a549-derived evs. (f) western blotting displays the expression of protein markers (cd63, cd9, tsg101, and calnexin) in hl60-derived evs.

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Caption : figure. s3  reusability of the dropfet. error bars represent standard deviations (n = 3).

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Caption : figure. s4  standard curves of mirna-21(a) and mirna-155(b) concentrations determined by qrt-pcr.

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Caption : figure. s5  qrt-pcr results of exosomal mirna-21 and mirna-155. (a) ct values of mirna-155 in a549 evs of normal cells and acute lung injury model. (b) ct values of mirna-155 in hl60 evs under normal, induced differentiation, and inflammatory models. (c) ct values of mirna-21 in a549 evs of normal cells and acute lung injury model. (d) ct values of mirna-21 in hl60 evs under normal, induced differentiation, and inflammatory models.

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Caption : figure. s6  cell states and bioactivity during the induction of differentiation and inflammation model of two cell lines. (a) a549 cells (a549_m1) after the construction of the acute lung injury model. (b) hl60 cells (hl60_m0) after induction of differentiation. (c) hl60 cells (hl60_m1) after the construction of the inflammation model.