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Caption : A) Fibroblasts on flat (top) and micropegged (bottom) substrates. (a-b) 1.79 MPa substrates, (c-d) 50 kPa substrates. F-actin. Scale bar, 25 μm. B) Cell area and cell shape index (CSI) did not significantly changed on the 1.79 MPa substrates compared to the 50 kPa substrates.

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Caption : (A) Fibroblasts on substrates with micropegs (P) have reduced expression of collagen type VI compared to fibroblasts cultures in (F) flat substrates as analyzed by western blot. Fibroblasts on substrates with micropegs have reduced expression (B) α-SMA, and (C) integrin α3 compared to fibroblasts cultures in flat substrates as analyzed by immonofluorescence staining. Data is normalized to 1.79 MPa flat substrate. Bars represent standard deviation and (*) indicates p < 0.05.

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Caption : Bright field imaging of cells at day 3 on flat and micropeg substrates (A-B) control, (C-D) with ROCK inhibitor (Y27632), (E-F) with MLCK inhibitor (ML7), and (G-H) with integrin α3 antibody. Scale bar, 25 μm.

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Caption : In the presence of (A) ROCK inhibitor (Y27632) or (B) myosin light chain kinase inhibitor (ML7) no difference in the expression of α-SMA,  is observed between cells cultured on flat substrates and cells cultured on micropegged substrates. (C) Blocking of integrin α3 results in down-regulation of α-SMA on the micropegged substrates compared to flat substrates as analyzed by immunofluorescence staining.  Data is normalized to 1.79 MPa flat substrate.  Bars represent standard deviation and (*) indicates p < 0.05.