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Caption : supplemental figure 1. kim-2 cells recapitulate activation of pstat3 in both 2d monoculture (a) and 2d trans-well co-culture (b) conditions. adipose was not found to have an effect on the level of pstat3 signalling in the model, although cleaved caspase 3 is more prevalent in the absence of differentiated 3t3-l1.

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Caption : supplemental fig 2. analysis of mmp gene expression during h/wd in 3d coculture model.

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Caption : supplemental fig 3. immunohistochemical cc3 quantification in ecad positive cells at the 48hr involution timepoint under conditions of h/wd, +/-osm and pan-caspase oph inhibitor q-vd (20μm). additionally caspase was quantified under conditions of serum withdrawal in the presence of lactogenic prl stimulation. n>3 for all samples.

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Caption : supplemental fig 4. kim2 cells loaded with lysotracker ® red to monitor acidic lysosome compartment with increasing bafilomycin concentration. 

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Caption : supplemental fig 5. a) csf-1 and mcp-1 are regulated in 3t3-l1 with adipogenic conversion. b) raw 264.7 cells undergo m2 conversion with il-4 treatment.