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["Table 1.  Kinetic and thermodynamic parameters for the duplex-coil equilibrium of seven pairs of DNA oligomers."]
[null,"flow rate","Tm","Eon","Eoff","kon(52°C) a","koff(52°C) a","K(52°C) a","kon","koff","K",null,"ΔH°","ΔS°"]
[null,null,"°C","kcal mol-1","kcal mol-1","× 103 M-1 s-1","× 10-3 s-1","M-1",null,null,"(kon/koff)",null,"kcal mol-1","cal mol-1 K-1"]
["S1-S2","batchwise",43.6,-49,26,0.54,31,"1.7 × 103",540,0.031,17419.354838709678,null,-75,-207]
["(8 base pairs,","0.7 cm s-1",43.7,-45,35,0.68,42,"1.6 × 103",680,0.042,16190.476190476189,null,-80,-228]
["GC content: 100%)","1.7 cm s-1",44.1,-43,42,1.1,68,"1.6 × 103",1100,0.068,16176.470588235294,null,-85,-241]
[null,"2.3 cm s-1",44.6,-45,43,0.53,32,"1.6 × 103",530,0.032,16562.5,null,-88,-252]
[null,"3.3 cm s-1",44.9,-47,49,0.81,48,"1.7 × 103",810,0.048,16875,null,-96,-277]
[null,"4.2 cm s-1",45.4,-43,54,1.5,89,"1.7 × 103",1500,0.089,16853.932584269663,null,-97,-280]
[null,"5.0 cm s-1",46,-45,54,1,49,"2.1 × 103",1000,0.049,20408.16326530612,null,-99,-283]
[null,"5.8 cm s-1",47.1,-46,60,1,36,"2.9 × 103",1000,0.036,27777.77777777778,null,-106,-310]
[null,"6.7 cm s-1",47.5,-44,63,1.5,34,"4.3 × 103",1500,0.034,44117.647058823524,null,-107,-309]
["S3-S4","batchwise",48.2,-47,23,6.4,51,"1.3 × 105",6400,0.051,125490.19607843139,null,-70,-186]
["(10 base pairs,","0.7 cm s-1",48,-47,25,3.9,38,"1.1 × 105",3900,0.038,102631.57894736843,null,-72,-195]
["GC content: 90%)","1.7 cm s-1",50.1,-45,28,1.5,11,"1.3 × 105",1500,0.011,136363.63636363638,null,-73,-202]
[null,"2.3 cm s-1",49,-46,36,2.9,22,"1.3 × 105",2900,0.022,131818.18181818182,null,-82,-226]
[null,"3.3 cm s-1",50.6,-45,47,1.7,7.2,"2.4 × 105",1700,0.0072,236111.11111111112,null,-92,-256]
[null,"4.2 cm s-1",52.1,-47,58,2.9,6.9,"4.2 × 105",2900,0.006900000000000001,420289.85507246375,null,-105,-301]
[null,"5.0 cm s-1",54.4,-44,64,5.3,3.9,"1.4 × 106",5300,0.0039,1358974.358974359,null,-108,-307]
[null,"5.8 cm s-1",54.5,-49,65,5.1,3.1,"1.6 × 106",5100,0.0031,1645161.2903225806,null,-114,-325]
[null,"6.7 cm s-1",56.8,-48,75,12,1.6,"7.1 × 106",12000,0.0016,7500000,null,-123,-356]
["S5-S6","batchwise",48.8,-59,20,7.2,4.3,"1.6 × 105",7200,0.0043,1674418.6046511629,null,-93,-265]
["(12 base pairs,","0.7 cm s-1",48.6,-52,43,2.3,16,"1.5 × 105",2300,0.016,143750,null,-107,-304]
["GC content: 75%)","1.7 cm s-1",49.8,-49,53,3.1,14,"2.2 × 105",3100,0.014,221428.57142857142,null,-114,-326]
[null,"2.3 cm s-1",51.4,-47,59,2.4,6.3,"3.7 × 105",2400,0.0063,380952.38095238095,null,-116,-331]
[null,"3.3 cm s-1",53,-40,54,3.4,4.6,"7.4 × 105",3400,0.0046,739130.4347826088,null,-111,-314]
[null,"4.2 cm s-1",54,-48,70,5.6,5.3,"1.1 × 106",5600,0.0053,1056603.7735849055,null,-112,-317]
[null,"5.0 cm s-1",55.6,-43,72,5.3,2.5,"2.1 × 106",5300,0.0025,2120000,null,-102,-283]
[null,"5.8 cm s-1",57.1,-45,63,12,2.8,"4.3 × 106",12000,0.0028,4285714.285714285,null,-107,-298]
[null,"6.7 cm s-1",59.1,-44,67,11,0.79,"1.4 × 107",11000,0.00079,13924050.632911392,null,-108,-298]
["S7-S8","batchwise",50.8,-64,29,6.3,31,"2.0 × 105",6300,0.031,203225.8064516129,null,-93,-265]
["(14 base pairs,","0.7 cm s-1",50.5,-64,43,1.9,11,"1.7 × 105",1900,0.011,172727.27272727274,null,-107,-304]
["GC content: 64%)","1.7 cm s-1",51.7,-62,52,1.9,9,"2.1 × 105",1900,0.009,211111.11111111112,null,-114,-326]
[null,"2.3 cm s-1",52.7,-56,60,2,4.7,"4.1 × 105",2000,0.0047,425531.914893617,null,-116,-331]
[null,"3.3 cm s-1",54.4,-50,61,2,2.5,"7.8 × 105",2000,0.0025,800000,null,-111,-314]
[null,"4.2 cm s-1",55.9,-48,64,2.8,1.5,"1.9 × 106",2800,0.0015,1866666.6666666665,null,-112,-317]
[null,"5.0 cm s-1",57.2,-43,59,4.1,1.1,"3.6 × 106",4100,0.0011,3727272.727272727,null,-102,-283]
[null,"5.8 cm s-1",59.5,-45,62,11,0.94,"1.2 × 107",11000,0.00094,11702127.659574468,null,-107,-298]
[null,"6.7 cm s-1",61,-41,67,16,0.48,"3.4 × 107",16000,0.00047999999999999996,33333333.333333336,null,-108,-298]
["S9-S10","batchwise",52.9,-66,37,6.1,1.1,"5.5 × 105",6100,0.0011,5545454.545454545,null,-103,-288]
["(17 base pairs,","0.7 cm s-1",52.8,-57,47,5.3,9.1,"5.8 × 105",5300,0.0091,582417.5824175824,null,-104,-298]
["GC content: 59%)","1.7 cm s-1",54.7,-63,60,3.1,3.4,"9.1 × 105",3100,0.0034,911764.705882353,null,-123,-352]
[null,"2.3 cm s-1",56.4,-55,71,3.8,1.4,"2.6 × 106",3800,0.0014,2714285.714285714,null,-126,-359]
[null,"3.3 cm s-1",58.1,-54,71,5.2,0.87,"6.0 × 106",5200,0.00087,5977011.494252874,null,-125,-354]
[null,"4.2 cm s-1",59.9,-41,77,5.7,0.42,"1.4 × 107",5700,0.00041999999999999996,13571428.571428573,null,-118,-330]
[null,"5.0 cm s-1",61.5,-38,78,7.2,0.21,"3.4 × 107",7200,0.00020999999999999998,34285714.28571429,null,-116,-321]
[null,"5.8 cm s-1",63.2,-39,80,12,0.11,"1.1 × 108",12000,0.00011,109090909.0909091,null,-119,-328]
[null,"6.7 cm s-1",65.2,-40,81,18,0.049,"3.6 × 108",18000,4.9000000000000005e-05,367346938.7755102,null,-121,-330]
["S11-S12","batchwise",53.7,-53,38,1.3,3.6,"3.6 × 105",1300,0.0036,361111.1111111111,null,-91,-248]
["(20 base pairs,","0.7 cm s-1",53.6,-30,38,1.2,3.3,"3.6 × 105",1200,0.0033,363636.36363636365,null,-68,-188]
["GC content: 55%)","1.7 cm s-1",56,-27,46,1.6,1.8,"8.8 × 105",1600,0.0018,888888.8888888889,null,-73,-198]
[null,"2.3 cm s-1",58,-28,49,1.1,0.73,"1.5 × 106",1100,0.00073,1506849.3150684931,null,-77,-207]
[null,"3.3 cm s-1",59.9,-19,40,1.7,0.6,"2.8 × 106",1700,0.0006,2833333.3333333335,null,-59,-155]
[null,"4.2 cm s-1",61.5,-17,45,2.1,0.44,"4.8 × 106",2100,0.00044,4772727.2727272725,null,-62,-160]
[null,"5.0 cm s-1",63,-20,64,1.3,0.51,"2.6 × 106",1300,0.00051,2549019.607843137,null,-84,-230]
[null,"5.8 cm s-1",64.5,-21,59,1.9,0.095,"2.0 × 107",1900,9.5e-05,20000000,null,-80,-212]
[null,"6.7 cm s-1",65.5,-16,62,2.2,0.074,"3.0 × 107",2200,7.4e-05,29729729.72972973,null,-78,-204]
["S13-S14","batchwise",53.3,-47,43,1.1,3.9,"2.8 × 105",1100,0.0039,282051.28205128206,null,-90,-249]
["(23 base pairs,","0.7 cm s-1",53.3,-27,51,0.87,3.2,"2.7 × 105",870,0.0032,271875,null,-78,-211]
["GC content: 48%)","1.7 cm s-1",55.5,-25,53,1.1,1.6,"7.1 × 105",1100,0.0016,687500,null,-78,-209]
[null,"2.3 cm s-1",57.1,-19,52,1,1.1,"9.0 × 105",1000,0.0011,909090.9090909091,null,-71,-195]
[null,"3.3 cm s-1",58.6,-17,60,0.86,0.51,"1.7 × 106",860,0.00051,1686274.5098039214,null,-77,-207]
[null,"4.2 cm s-1",60.4,-12,56,1.1,0.36,"3.0 × 107",1100,0.00035999999999999997,3055555.555555556,null,-68,-183]
[null,"5.0 cm s-1",61.8,-11,57,1,0.23,"4.4 × 107",1000,0.00023,4347826.0869565215,null,-68,-182]
[null,"5.8 cm s-1",62.9,-12,67,1.2,0.13,"8.9 × 107",1200,0.00013000000000000002,9230769.23076923,null,-79,-212]
[null,"6.7 cm s-1",64,-13,70,1.2,0.071,"1.7 × 108",1200,7.099999999999999e-05,16901408.450704228,null,-83,-219]
["Estimated errors:  Tm ,<  ±0.3°C;  Eon and  Eoff, < 10%;  kon(52°C) and  koff(52°C) , < 15%;  K(52°C), < 20%; ΔH° and ΔS°, < 10%."]
["[a] These values are at 52°C."]