{"text_format":"2","workbook":"xls","n_sheets":7,"sheets":["S1 (MaLi) - Table 1","S2 - Table 1","S3 - Table 1","S4 - Table 1","S5 - Table 1","S6 - Table 1","S7 - Table 1"],"not_rendered":{"charts":0,"images":0,"macros":0,"external_links":0,"defined_names":0,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"run_cost":"one","rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"S1 (MaLi) - Table 1","index":0,"n_rows":225,"n_cols":5,"rows_kept":225,"cut":false,"hidden":false,"merged":[],"flags":["formulas_cached_only"]}
["Table S1 Reactants of the reconstruction of the M. pneumoniae metabolic network"]
["Metabolite","Identified"]
["(S)-Lactate","Y",null,"Y","identified experimentally"]
["3-Phospho-D-glycerate","Y",null,"N","not identified"]
["Acetate","Y",null,"O","not predicted, newly identified experimentally"]
["Adenine","Y",null,"R","not amenable for experimental analysis"]
["Adenosine","Y"]
["ADP","Y"]
["AMP","Y"]
["ATP","Y"]
["CDP","Y"]
["Choline","Y"]
["CMP","Y"]
["CTP","Y"]
["dADP","Y"]
["dAMP","Y"]
["dATP","Y"]
["D-Fructose","Y"]
["D-Fructose 1,6-bisphosphate","Y"]
["D-Fructose 6-phosphate","Y"]
["dGDP","Y"]
["D-Glucose","Y"]
["D-Glucose 1-phosphate","Y"]
["D-Glucose 6-phosphate","Y"]
["D-Glyceraldehyde 3-phosphate","Y"]
["dGMP","Y"]
["dGTP","Y"]
["Dihydroxyacetone phosphate","Y"]
["D-Mannitol 1-phosphate","Y"]
["D-Ribose","Y"]
["D-Ribose 5-phosphate","Y"]
["dTDP","Y"]
["dTMP","Y"]
["dTTP","Y"]
["FAD","Y"]
["Fatty Acid","Y"]
["Formate","Y"]
["GDP","Y"]
["Glycerol","Y"]
["Glycine","Y"]
["GMP","Y"]
["GTP","Y"]
["Guanine","Y"]
["Guanosine","Y"]
["L-Alanine","Y"]
["L-Arginine","Y"]
["L-Asparagine","Y"]
["L-Aspartate","Y"]
["L-Cysteine","Y"]
["L-Glutamate","Y"]
["L-Glutamine","Y"]
["L-Histidine","Y"]
["L-Isoleucine","Y"]
["L-Leucine","Y"]
["L-Lysine","Y"]
["L-Methionine","Y"]
["L-Ornithine","Y"]
["L-Phenylalanine","Y"]
["L-Proline","Y"]
["L-Serine","Y"]
["L-Threonine","Y"]
["L-Tryptophan","Y"]
["L-Tyrosine","Y"]
["L-Valine","Y"]
["NAD+","Y"]
["NADH","Y"]
["NADP+","Y"]
["NADPH","Y"]
["Phosphoenolpyruvate","Y"]
["Pyruvate","Y"]
["Riboflavin","Y"]
["sn-Glycerol 3-phosphate","Y"]
["Thiamin diphosphate","Y"]
["Thymine","Y"]
["UDP","Y"]
["UDP-D-galactose","Y"]
["UDP-glucose","Y"]
["UMP","Y"]
["Uracil","Y"]
["Uridine","Y"]
["UTP","Y"]
["ADP-GLUCOSE","O"]
["CHOLESTEROL","O"]
["CYTOSINE","O"]
["ETHANOL","O"]
["FUMARATE","O"]
["SUCCINATE","O"]
["trans-4-hydroxy-L-proline","O"]
["1,3-Bisphospho-D-glycerate","N","IP-RP",null,"I put info behind the metabolites for which we could measure a standard with the dedicated method"]
["10-Formyltetrahydrofolate","N","IP-RP"]
["1-acyl-Glycerol 3-phosphate","N","IP-RP"]
["2-Deoxy-D-ribose 1-phosphate","N","IP-RP"]
["2-Deoxy-D-ribose 5-phosphate","N","IP-RP"]
["2-Phospho-D-glycerate","N","IP-RP","HILIC"]
["3-keto-L-gulonate 6-phosphate","N"]
["5,10-Methenyltetrahydrofolate","N"]
["5,10-Methylenetetrahydrofolate","N","IP-RP","RP"]
["5-Formyltetrahydrofolate","N"]
["5-Phospho-alpha-D-ribose 1-diphosphate","N"]
["Acetaldehyde","N"]
["Acetyl phosphate","N"]
["Acetyl-CoA","N","IP-RP","RP"]
["Adenosine 3',5'-bisphosphate","N"]
["Carbamoyl phosphate","N"]
["CDP-choline","N"]
["CDP-diacylglycerol","N"]
["Choline phosphate","N"]
["CoA","N","IP-RP","RP"]
["Cytidine","N"]
["dCDP","N","IP-RP"]
["dCMP","N","IP-RP"]
["dCTP","N","IP-RP"]
["Deamino-NAD+","N"]
["Deoxyadenosine","N"]
["Deoxycytidine","N"]
["Deoxyguanosine","N"]
["Deoxyuridine","N"]
["Dephospho-CoA","N"]
["D-Erythrose 4-phosphate","N"]
["D-Fructose 1-phosphate","N"]
["D-Glyceraldehyde","N"]
["Diacylglycerol","N"]
["Dihydrofolate","N"]
["Dihydrolipoamide","N"]
["D-Mannose","N"]
["D-Mannose 6-phosphate","N"]
["D-Ribose 1-phosphate","N"]
["D-Ribulose 5-phosphate","N"]
["D-Xylulose 5-phosphate","N"]
["FMN","Y"]
["Folic acid","N"]
["Glycerone","N"]
["L-Ascorbate","N"]
["L-ascorbate 6-phosphate","N"]
["L-Citrulline","N"]
["L-Homocysteine","N"]
["Lipoamide","N"]
["L-Ribulose 5-phosphate","N","HILIC","RP"]
["L-Xylulose 5-phosphate","N"]
["Mannitol","N"]
["Nicotinate","N"]
["Nicotinate D-ribonucleotide","N"]
["Pantetheine","N"]
["Pantetheine 4-phosphate","N"]
["phosphatidic acid","N"]
["Phosphatidylcholine","N"]
["phosphatidylglycerol","N"]
["phosphatidylglycerol 3-phosphate","N"]
["Pyridoxal","N"]
["Pyridoxal phosphate","Y"]
["S-Acetyldihydrolipoamide","N"]
["S-Adenosyl-L-homocysteine","N"]
["S-Adenosyl-L-methionine","N"]
["Sedoheptulose 7-phosphate","N","HILIC","RP"]
["sn-Glycero-3-phosphocholine","N"]
["Tetrahydrofolate","N"]
["Thiamin","N"]
["Thiamin monophosphate","N"]
["Thymidine","N"]
["5mcDNA (Mpn)","R"]
["ACP-R (Mpn)","R"]
["acyl carrier protein","R"]
["apoprotein [acyl carrier protein]","R"]
["Biomass","R"]
["Cardiolipin (Mpn)","R"]
["CO2","R"]
["DNA (Mpn)","R"]
["DnaK-folded protein","R"]
["dUDP","R","IP-RP"]
["dUMP","R","IP-RP"]
["glycolipid (Mpn)","R"]
["Glycyl-tRNA(Gly)","R"]
["GroEL folded protein","R"]
["H+","R"]
["H2O","R"]
["H2O2","R"]
["L-Alanyl-tRNA(Ala)","R"]
["L-Arginyl-tRNA(Arg)","R"]
["L-Asparaginyl-tRNA(Asn)","R"]
["L-Aspartyl-tRNA(Asp)","R"]
["L-Cysteinyl-tRNA(Cys)","R"]
["L-Glutaminyl-tRNA(Gln)","R"]
["L-Glutamyl-tRNA(Gln)","R"]
["L-Glutamyl-tRNA(Glu)","R"]
["L-Histidyl-tRNA(His)","R"]
["L-Isoleucyl-tRNA(Ile)","R"]
["L-Leucyl-tRNA(Leu)","R"]
["L-Lysyl-tRNA(Lys)","R"]
["L-Methionyl-tRNA(Met)","R"]
["L-Phenylalanyl-tRNA(Phe)","R"]
["L-Prolyl-tRNA(Pro)","R"]
["L-Seryl-tRNA(Ser)","R"]
["L-Threonyl-tRNA(Thr)","R"]
["L-Tryptophanyl-tRNA(Trp)","R"]
["L-Tyrosyl-tRNA(Tyr)","R"]
["L-Valyl-tRNA(Val)","R"]
["N-Formylmethionyl-tRNA(Met)","R"]
["NH3","R"]
["Orthophosphate","R"]
["Oxidized thioredoxin","R"]
["Oxygen","R"]
["Protein (Mpn)","R"]
["Pyrophosphate","R"]
["Reduced thioredoxin","R"]
["RNA (Mpn)","R"]
["tRNA(Ala)","R"]
["tRNA(Arg)","R"]
["tRNA(Asn)","R"]
["tRNA(Asp)","R"]
["tRNA(Cys)","R"]
["tRNA(Gln)","R"]
["tRNA(Glu)","R"]
["tRNA(Gly)","R"]
["tRNA(His)","R"]
["tRNA(Ile)","R"]
["tRNA(Leu)","R"]
["tRNA(Lys)","R"]
["tRNA(Met)","R"]
["tRNA(Phe)","R"]
["tRNA(Pro)","R"]
["tRNA(Ser)","R"]
["tRNA(Thr)","R"]
["tRNA(Trp)","R"]
["tRNA(Tyr)","R"]
["tRNA(Val)","R"]

{"sheet":"S2 - Table 1","index":1,"n_rows":88,"n_cols":8,"rows_kept":88,"cut":false,"hidden":false,"merged":[],"flags":["formulas_cached_only"]}
["Table S2. Experimentally identified metabolites in this study using GC-MS, LC-MS and NMR"]
["Metabolite name","KEGG ID","LC-MS","LC-MS method","ionization mode","mass error [ppm]","GC-MS","NMR"]
["D-fructose 1,6-bisphosphate","C00354","Y","IP-RP","neg",4,"Y"]
["D-glucose 1-phosphate","C00103","Y","IP-RP","neg",6,"Y"]
["D-glucose 6-phosphate","C00092","Y","IP-RP","neg",1,"Y"]
["glycerol 3-phosphate (sn-Glycerol 3-phosphate)","C00093","Y","IP-RP","neg",4,"Y"]
["ADP","C00008","Y","IP-RP","neg",8,null,"Y"]
["ATP","C00002","Y","IP-RP","neg",2,null,"Y"]
["NAD+","C00003","Y","IP-RP","neg",7,null,"Y"]
["UDP-D-galactose","C00052","Y","IP-RP","neg",3,null,"Y"]
["UDP-D-glucose","C00029","Y","IP-RP","neg",2,null,"Y"]
["3-phospho-D-glycerate","C00197","Y","IP-RP","neg",3]
["ADP-glucose","C00498","Y","IP-RP","neg",3]
["AMP","C00020","Y","IP-RP","neg",4]
["CDP","C00112","Y","IP-RP","neg",7]
["CMP","C00055","Y","IP-RP","neg",7]
["CTP","C00063","Y","IP-RP","neg",1]
["dADP","C00206","Y","IP-RP","neg",7]
["dAMP","C00360","Y","IP-RP","neg",7]
["dATP","C00131","Y","IP-RP","neg",3]
["dGDP","C00361","Y","IP-RP","neg",9]
["dGMP","C00362","Y","IP-RP","neg",8]
["dGTP","C00286","Y","IP-RP","neg",2]
["D-mannitol 1-phosphate","C00644","Y","IP-RP","neg",3]
["FAD","C00016","Y","IP-RP","neg",8]
["GDP","C00035","Y","IP-RP","neg",5]
["GMP","C00144","Y","IP-RP","neg",7]
["GTP","C00044","Y","IP-RP","neg",2]
["NADH","C00004","Y","IP-RP","neg",6]
["NADP+","C00006","Y","IP-RP","neg",3]
["NADPH","C00005","Y","IP-RP","neg",8]
["riboflavin","C00255","Y","IP-RP","neg",6]
["thiamine diphosphate","C00068","Y","IP-RP","neg",2]
["thymidine-5'-diphosphate","C00363","Y","IP-RP","neg",1]
["thymidine-5'-phosphate","C00364","Y","IP-RP","neg",5]
["thymidine-5'-triphosphate","C00459","Y","IP-RP","neg",3]
["UDP","C00015","Y","IP-RP","neg",9]
["UMP","C00105","Y","IP-RP","neg",4]
["UTP","C00075","Y","IP-RP","neg",3]
["Arginine","C00062",{"$nulls":4},"Y","Y"]
["Aspartate","C00049",{"$nulls":4},"Y","Y"]
["Glutamate","C00025",{"$nulls":4},"Y","Y"]
["Glycine","C00037",{"$nulls":4},"Y","Y"]
["Histidine","C00135",{"$nulls":4},"Y","Y"]
["Isoleucine","C00407",{"$nulls":4},"Y","Y"]
["Leucine","C00123",{"$nulls":4},"Y","Y"]
["Lysine","C00047",{"$nulls":4},"Y","Y"]
["Methionine","C00073",{"$nulls":4},"Y","Y"]
["Phenylalanine","C00079",{"$nulls":4},"Y","Y"]
["Proline","C00148",{"$nulls":4},"Y","Y"]
["Serine","C00065",{"$nulls":4},"Y","Y"]
["Threonine","C00188",{"$nulls":4},"Y","Y"]
["Tryptophane","C00078",{"$nulls":4},"Y","Y"]
["Tyrosine","C00082",{"$nulls":4},"Y","Y"]
["Valine","C00183",{"$nulls":4},"Y","Y"]
["glycerol","C00116",{"$nulls":4},"Y"]
["fructose","C00095",{"$nulls":4},"Y"]
["Adenine","C00147",{"$nulls":4},"Y"]
["Alanine","C00041",{"$nulls":4},"Y"]
["Asparagine","C00152",{"$nulls":4},"Y"]
["Cholesterol","C00187",{"$nulls":4},"Y"]
["Cysteine","C00097",{"$nulls":4},"Y"]
["Cytosine","C00380",{"$nulls":4},"Y"]
["DHAP","C00111",{"$nulls":4},"Y"]
["Fatty acids (various)","N/A",{"$nulls":4},"Y"]
["Fru6P","C00085",{"$nulls":4},"Y"]
["GAP","C00118",{"$nulls":4},"Y"]
["Glucose","C00031",{"$nulls":4},"Y"]
["Glutamine","C00064",{"$nulls":4},"Y"]
["Guanine","C00242",{"$nulls":4},"Y"]
["PEP","C00074",{"$nulls":4},"Y"]
["ribose","C00121",{"$nulls":4},"Y"]
["Ribose-5-phosphate","C00199",{"$nulls":4},"Y"]
["Thymine","C00178",{"$nulls":4},"Y"]
["Uracil","C00106",{"$nulls":4},"Y"]
["adenosine","C00212",{"$nulls":4},"Y"]
["guanosine","C00387",{"$nulls":4},"Y"]
["uridine","C00299",{"$nulls":4},"Y"]
["(S)-lactate","C00186",{"$nulls":5},"Y"]
["acetate","C00033",{"$nulls":5},"Y"]
["choline","C00114",{"$nulls":5},"Y"]
["ethanol","C00469 ",{"$nulls":5},"Y"]
["formate","C00058",{"$nulls":5},"Y"]
["fumarate","C00122 ",{"$nulls":5},"Y"]
["Ornithine","C00077 ",{"$nulls":5},"Y"]
["pyruvate","C00022",{"$nulls":5},"Y"]
["succinate","C00042 ",{"$nulls":5},"Y"]
["trans-4-hydroxy-L-proline","C03651",{"$nulls":5},"Y"]

{"sheet":"S3 - Table 1","index":2,"n_rows":161,"n_cols":8,"rows_kept":161,"cut":false,"hidden":false,"merged":[],"flags":["formulas_cached_only"]}
["Table S3. Integration of the in silico predicted M. pneumoniae metabolites with experimentally identified metabolites in this study, in large-scale studies with other organisms, or using purifies standard compounds."]
["in silico predicted Metabolite (Wodke et al. model)","This study exerimental","Other studies experimental","other studies standards",null,null,"Y","identified experimentally"]
["Adenosine","Y","Y","Y",null,null,"N","not identified"]
["Guanosine","Y","Y","Y",null,null,"O","not predicted, newly identified experimentally"]
["Uridine","Y","N","Y"]
["Glycerol","Y","Y","Y"]
["dTDP","Y","Y","Y"]
["dTMP","Y","N","Y"]
["dTTP","Y","Y","Y"]
["(S)-Lactate","Y","Y","Y"]
["3-Phospho-D-glycerate","Y","Y","Y"]
["Acetate","Y","Y","Y"]
["Adenine","Y","Y","Y"]
["ADP","Y","Y","Y"]
["AMP","Y","Y","Y"]
["ATP","Y","Y","Y"]
["CDP","Y","Y","Y"]
["Choline","Y","N","Y"]
["CMP","Y","Y","Y"]
["CTP","Y","Y","Y"]
["dADP","Y","Y","Y"]
["dAMP","Y","Y","Y"]
["dATP","Y","Y","Y"]
["D-Fructose","Y","Y","Y"]
["D-Fructose 1,6-bisphosphate","Y","Y","Y"]
["D-Fructose 6-phosphate","Y","Y","Y"]
["dGDP","Y","N","Y"]
["D-Glucose","Y","Y","Y"]
["D-Glucose 1-phosphate","Y","N","Y"]
["D-Glucose 6-phosphate","Y","Y","Y"]
["D-Glyceraldehyde 3-phosphate","Y","N","Y"]
["dGMP","Y","N","Y"]
["dGTP","Y","N","Y"]
["Dihydroxyacetone phosphate","Y","Y","Y"]
["D-Mannitol 1-phosphate","Y","N","Y"]
["D-Ribose","Y","N","Y"]
["D-Ribose 5-phosphate","Y","Y","Y"]
["FAD","Y","Y","Y"]
["Fatty Acid","Y","Y","Y"]
["Formate","Y","Y","Y"]
["GDP","Y","Y","Y"]
["Glycine","Y","Y","Y"]
["GMP","Y","Y","Y"]
["GTP","Y","Y","Y"]
["Guanine","Y","Y","Y"]
["L-Alanine","Y","Y","Y"]
["L-Arginine","Y","Y","Y"]
["L-Asparagine","Y","Y","Y"]
["L-Aspartate","Y","Y","Y"]
["L-Cysteine","Y","Y","Y"]
["L-Glutamate","Y","Y","Y"]
["L-Glutamine","Y","Y","Y"]
["L-Histidine","Y","Y","Y"]
["L-Isoleucine","Y","Y","Y"]
["L-Leucine","Y","Y","Y"]
["L-Lysine","Y","Y","Y"]
["L-Methionine","Y","Y","Y"]
["L-Ornithine","Y","Y","Y"]
["L-Phenylalanine","Y","Y","Y"]
["L-Proline","Y","Y","Y"]
["L-Serine","Y","Y","Y"]
["L-Threonine","Y","Y","Y"]
["L-Tryptophan","Y","Y","Y"]
["L-Tyrosine","Y","Y","Y"]
["L-Valine","Y","Y","Y"]
["NAD+","Y","Y","Y"]
["NADH","Y","Y","Y"]
["NADP+","Y","Y","Y"]
["NADPH","Y","Y","Y"]
["Phosphoenolpyruvate","Y","Y","Y"]
["Pyruvate","Y","Y","Y"]
["Riboflavin","Y","N","Y"]
["sn-Glycerol 3-phosphate","Y","Y","Y"]
["Thiamin diphosphate","Y","N","Y"]
["Thymine","Y","Y","Y"]
["UDP","Y","Y","Y"]
["UDP-D-galactose","Y","Y","Y"]
["UDP-glucose","Y","Y","Y"]
["UMP","Y","Y","Y"]
["Uracil","Y","Y","Y"]
["UTP","Y","Y","Y"]
["CHOLESTEROL","O","N","Y"]
["ADP-GLUCOSE","O","Y","Y"]
["CYTOSINE","O","N","Y"]
["ETHANOL","O","Y","Y"]
["FUMARATE","O","Y","Y"]
["SUCCINATE","O","Y","Y"]
["trans-4-hydroxy-L-proline","O","N","Y"]
["1,3-Bisphospho-D-glycerate","N","N","N"]
["10-Formyltetrahydrofolate","N","N","N"]
["1-acyl-Glycerol 3-phosphate","N","N","Y"]
["2-Deoxy-D-ribose 1-phosphate","N","N","N"]
["2-Deoxy-D-ribose 5-phosphate","N","N","Y"]
["2-Phospho-D-glycerate","N","Y","Y"]
["3-keto-L-gulonate 6-phosphate","N","N","Y"]
["5,10-Methenyltetrahydrofolate","N","N","N"]
["5,10-Methylenetetrahydrofolate","N","N","N"]
["5-Formyltetrahydrofolate","N","N","N"]
["5-Phospho-alpha-D-ribose 1-diphosphate","N","N","N"]
["Acetaldehyde","N","N","N"]
["Acetyl phosphate","N","N","Y"]
["Acetyl-CoA","N","Y","Y"]
["Adenosine 3',5'-bisphosphate","N","N","Y"]
["Carbamoyl phosphate","N","N","N"]
["CDP-choline","N","N","N"]
["CDP-diacylglycerol","N","N","Y"]
["Choline phosphate","N","N","Y"]
["CoA","N","Y","Y"]
["Cytidine","N","Y","Y"]
["dCDP","N","Y","Y"]
["dCMP","N","N","Y"]
["dCTP","N","Y","Y"]
["Deamino-NAD+","N","N","Y"]
["Deoxyadenosine","N","N","Y"]
["Deoxycytidine","N","N","Y"]
["Deoxyguanosine","N","N","Y"]
["Deoxyuridine","N","N","Y"]
["Dephospho-CoA","N","N","Y"]
["D-Erythrose 4-phosphate","N","Y","Y"]
["D-Fructose 1-phosphate","N","N","Y"]
["D-Glyceraldehyde","N","N","Y"]
["Diacylglycerol","N","N","N"]
["Dihydrofolate","N","N","Y"]
["Dihydrolipoamide","N","N","N"]
["D-Mannose","N","N","Y"]
["D-Mannose 6-phosphate","N","N","Y"]
["D-Ribose 1-phosphate","N","N","Y"]
["D-Ribulose 5-phosphate","N","Y","Y"]
["D-Xylulose 5-phosphate","N","N","N"]
["FMN","N","Y","Y"]
["Folic acid","N","N","Y"]
["Glycerone","N","N","Y"]
["L-ascorbate 6-phosphate","N","N","Y"]
["L-Citrulline","N","Y","Y"]
["L-Homocysteine","N","N","Y"]
["Lipoamide","N","N","Y"]
["L-Ribulose 5-phosphate","N","Y","Y"]
["L-Xylulose 5-phosphate","N","N","Y"]
["Mannitol","N","N","Y"]
["Nicotinate","N","Y","Y"]
["Nicotinate D-ribonucleotide","N","N","N"]
["Pantetheine","N","N","Y"]
["Pantetheine 4-phosphate","N","N","N"]
["phosphatidic acid","N","N","N"]
["phosphatidylglycerol","N","Y","N"]
["phosphatidylglycerol 3-phosphate","N","N","N"]
["Phosphatidylcholine","N","N","N"]
["Pyridoxal","N","N","Y"]
["Pyridoxal phosphate","N","Y","Y"]
["S-Acetyldihydrolipoamide","N","N","N"]
["S-Adenosyl-L-homocysteine","N","N","Y"]
["S-Adenosyl-L-methionine","N","N","Y"]
["Sedoheptulose 7-phosphate","N","Y","Y"]
["sn-Glycero-3-phosphocholine","N","N","N"]
["Tetrahydrofolate","N","Y","Y"]
["Thiamin","N","N","N"]
["Thiamin monophosphate","N","N","Y"]
["Thymidine","N","N","Y"]
["L-Ascorbate","N","N","Y"]
["dUDP","N","N","N"]
["dUMP","N","N","Y"]

{"sheet":"S4 - Table 1","index":3,"n_rows":25,"n_cols":7,"rows_kept":25,"cut":false,"hidden":false,"merged":[],"flags":["formulas_cached_only"]}
["Table S4. Quantitative amino acid analysis of the proteome and the cytosol"]
["aa",null,"aa in proteome","aa in cytosol","times enrichment","import/min during exp growth","min for intracellular pool tunrover"]
["L","Leu",2393036,25097.05592913839,95.3512637799726,1994.1966666666667,12.585045571803377]
["E","Glu",1672072,98709.27690427659,16.93936023481859,1393.3933333333334,70.84092807315228]
["A","Ala",2032737,38012.458206949574,53.47554712019039,1693.9475,22.440163114234398]
["V","Val",1864124,12050.334305584664,154.69479540796488,1553.4366666666667,7.75720991023215]
["I","Ile",1724690,17162.645565208353,100.49091752475763,1437.2416666666666,11.941377684250517]
["S","Ser",1515135,12566.026347110956,120.57391558377111,1262.6125,9.952401348086571]
["G","Gly",1588085,34936.658649543664,45.4561214891895,1323.4041666666667,26.39908467081573]
["D","Asp",1305481,23289.714936892153,56.053970756509706,1087.9008333333334,21.407939237928844]
["T","Thr",1533247,6433.410380326506,238.32569498266412,1277.7058333333334,5.035126405850986]
["F","Phe",1102859,39005.54349337493,28.27441694761464,919.0491666666666,42.44119347264693]
["R","Arg",907229,38302.24724840229,23.686051476727478,756.0241666666666,50.66272870254671]
["P","Pro",974850,35012.34123028322,27.843039503934204,812.375,43.09874285925]
["N","Asn",1473974,13655.645188364413,107.93880330575162,1228.3116666666667,11.117410636847932]
["M","Met",469166,12369.476786726444,37.92933267019484,390.97166666666664,31.637783096114667]
["Q","Gln",1179691,7692.708238618437,153.35184481296088,983.0758333333334,7.825142250252077]
["K","Lys",2149849,24800.00990495273,86.68742505504648,1791.5408333333332,13.842838211401489]
["H","His",478238,12279.469738303413,38.94614427105348,398.5316666666667,30.811779252096432]
["C","Cys",197940,3627.5517146154225,54.56572795433869,164.95,21.991826096486346]
["Y","Tyr",748700,5417.454387835065,138.20144045535696,623.9166666666666,8.682977514895256]
["W","Trp",211534,6470.8277604091345,32.69040806405659,176.27833333333334,36.708015319007636]
[]
["sum",null,25522637,466890.8569169164,80.57381106984371]
["average",{"$nulls":4},1063.4432083333334,24.35898567139502]

{"sheet":"S5 - Table 1","index":4,"n_rows":6,"n_cols":5,"rows_kept":6,"cut":false,"hidden":false,"merged":[],"flags":["formulas_cached_only"]}
["Table S5. Known permeases transporting nucleotides and their closest orthologs in M. pneumoniae"]
["Organism","Protein name","Closest M. pneumoniae ortholog ","p value","protein abundance"]
["E. coli","UraA ","MPN080",0.15,21]
["E. coli","PurB ","MPN657",0.31,"n.d."]
["E. coli","CodB ","MPN080",0.12,21]
["B. subtilis ","PyrB ","MPN475",0.19,16]

{"sheet":"S6 - Table 1","index":5,"n_rows":24,"n_cols":3,"rows_kept":24,"cut":false,"hidden":false,"merged":[],"flags":["formulas_cached_only"]}
["Table S6. Retention times (RT) and characteristic ions used for monitoring amino acids (AA). Quantitation based on underscored ions."]
["AA","RT (min)","Ions (m/z)"]
["Arg",6.89,"630, 573, 286"]
["Ala",3.65,"260, 232, 158"]
["Gly",3.77,"246, 218, 144"]
["Val",4.26,"288, 260, 186"]
["Leu",4.46,"302, 274, 200 "]
["Ile",4.62,"302, 274, 200"]
["Met",5.67,"320, 292, 218"]
["Ser",5.77,"390, 362, 288"]
["Thr",5.9,"404, 376, 303 "]
["Phe",6.17,"336, 302, 234"]
["Asp",6.43,"418, 390, 316 "]
["Cys",6.62,"406, 378, 304"]
["Glu",6.87,"432, 330, 272"]
["Pro",4.78,"286, 258, 184"]
["Asn",6.99,"417, 302, 315"]
["Lys",7.26,"431, 329, 300"]
["Gln",7.41,"431, 357, 329"]
["His",7.97,"440, 338, 196"]
["Tyr",8.14,"466, 364, 302"]
["Trp",8.94,"489, 302, 244"]
["Cis",9.77,"639, 537, 348"]
["nLeu (ISTD)",4.7,"302, 274, 200"]

{"sheet":"S7 - Table 1","index":6,"n_rows":14,"n_cols":3,"rows_kept":14,"cut":false,"hidden":false,"merged":[],"flags":["formulas_cached_only"]}
["Table S7. Retention times and characteristic ions for bases, nucleosides, and cholesterol after per-trimethylsilyl derivatisation. Underscored ions were used for quantitation."]
["Compound","RT (min)","Ions (m/z)"]
["Adenine",7.66,"279, 264, 192"]
["Thymine",5.33,"270, 255, 113"]
["Cytosine",6.03,"254, 240, 170"]
["Guanine",8.76,"367, 352, 280"]
["Uracil",4.94,"256, 241, 147"]
["Adenosine",10.56,"540, 322, 230"]
["Guanosine",11.02,"643, 410, 324"]
["Thymidine",10,"458, 353, 199"]
["Uridine",9.98,"517, 348, 217"]
["Cytidine",10.75,"516, 348, 223"]
["Cholesterol",12.07,"458, 368, 329"]
["nLeu (ISTD)",4.83,"260,232, 158"]