Techniques for Biomolecule Characterisation

4 October 2016 13:15-15:00, Cambridge, United Kingdom

Techniques for Biomolecule Characterisation
This short seminar discusses chromatographic techniques suitable for the analysis of large molecular weight biomolecules. With an increased interest in the area of biotherapeutics and more regulations focused on these pharmaceuticals, possible methods to characterise biomolecules are required.  Modes such as size exclusion (SEC), ion-exchange, hydrophobic interaction (HIC) and affinity chromatography will be covered for intact biomolecules. Techniques that may be more familiar to those analysts involved in small molecule analysis, such as reversed-phase LC-MS methods for protein digests will also be discussed, alongside new tools focused primarily on large molecules, for example SEC for monoclonal antibody monomer, fragment and aggregate separations. Course attendees will leave with a better understanding of the variety of techniques available to enable a more complete characterisation of biomolecules. 
Biomolecule basics
Size exclusion chromatography
Ion-exchange chromatography
Hydrophobic interaction chromatography
Reversed-phase chromatography
Affinity chromatography

Available as in-house or off-site courses. Contact us for details
Tel: 0118 930 3660 Email:
Trinity Centre Cambridge

Suite 1, Trinity Centre Cambridge, 24 Cambridge Science Park, Milton Road, Cambridge, CB4 0FN, United Kingdom

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Hichrom is one of the world’s leading HPLC column manufacturers and has been firmly established in the field of Liquid Chromatography for over 30 years, developing, manufacturing and supplying the highest quality LC columns throughout the world.  During this time Hichrom has built up a strong reputation amongst chromatographers for our technical expertise. Our speakers are experienced chromatographers who will be happy to discuss your questions on a wide variety of HPLC/UHPLC topics including: troubleshooting, method development, reproducibility, batch reservation, custom packing/bonding, application support, method transfer & column selection.

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