The Analysis of Polar Molecules

7 June 2016 11:15-13:00, Sunderland, United Kingdom

This course is suitable for analysts looking to explore different chromatographic techniques/modes applicable for the analysis of polar molecules. It will introduce you to a number of possible techniques that can be employed to retain polar molecules, generally hard to retain using C18 columns in reversed-phase solvent systems. It includes details of new column chemistries suitable for reversed-phase retention of polars, such as polar embedded columns, as well as alternative techniques including: ion pairing, mixed-mode, Hydrophilic Interaction Liquid Chromatography (HILIC), Aqueous Normal Phase (ANP) and Supercritical Fluid Chromatography (SFC). This course allows you to explore the various options available to expand the polarity range of molecules that can be retained and discusses how these complement standard reversed-phase methodology.   
Limitations of reversed-phase HPLC
Alternatives to C18
Ion pairing
Aqueous normal-phase
Available as in-house or off-site courses. Contact us for details
Tel: 0118 930 3660 Email:

Sunderland Marriott Hotel

Boardroom, Sunderland Marriott Hotel, Queens Parade, Sunderland, SR6 8DB, United Kingdom

Organised by
Hichrom is one of the world’s leading HPLC column manufacturers and has been firmly established in the field of Liquid Chromatography for over 30 years, developing, manufacturing and supplying the highest quality LC columns throughout the world.  During this time Hichrom has built up a strong reputation amongst chromatographers for our technical expertise. Our speakers are experienced chromatographers who will be happy to discuss your questions on a wide variety of HPLC/UHPLC topics including: troubleshooting, method development, reproducibility, batch reservation, custom packing/bonding, application support, method transfer & column selection.

Contact information
Showing all upcoming events
Start Date
End Date
Subject area
Event type


E-mail Enquiry