EMBL Course: Next Generation Sequencing: Amplicon Based Targeted Resequencing

13 - 16 February 2017, Heidelberg, Germany


Introduction
We will start with an introduction into Illumina NGS technology and the library preparation workflow. The main part will be the wet lab preparation of libraries from genomic DNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quality control and normalize the prepared libraries. We will also have a basic training on assay design using DesignStudio and data analysis of TruSeq Amplicon sequencing results using standard Illumina tools like MiSeq Reporter and VariantStudio.
Venue
EMBL ATC

EMBL ATC, Meyerhofstr 1, Meyerhofstr 1, Heidelberg, 69117, Germany

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