How to Develop HPLC Methods

24 November 2022 09:00 - 25 November 2022 15:00

Learn how to select appropriate method conditions and perform suitable investigative experiments to obtain a set of method parameters which enables the desired separation for mixtures of analytes. In doing so you will learn how HPLC methods work even if you never need to develop a new method.

This course will enable you to take a strategic approach to developing HPLC methods with an understanding of the factors which can be adjusted to manipulate the retention time of analytes.
Additionally, learn how to implement strategies to achieve satisfactory separation for ‘complex’ samples and to find solutions for difficult HPLC separations in order to develop robust and fit for purpose HPLC methods.

Learning Objectives

  1. Define the objectives for the development of a HPLC analytical method.
  2. Effectively assess all the available relevant information relating to the desired method, e.g., pKa of the analyte.
  3. Select and prepare a suitable sample or samples to be used for the method development.
  4. Select suitable scouting conditions to find a suitable column and mobile phase system.
  5. Optimise the chromatographic conditions to result in the best possible separation.
  6. Apply strategies to achieve satisfactory separations for ‘complex’ samples with respect to: Optimising gradient methods, Sample preparation, Detection methods, Retaining very polar analytes, Selecting columns.
This course focuses on reversed phase mode separations.

Attendees are invited to bring along any real life examples that they would like advice on during the training. These may be discussed during group exercises, or, where intellectual property is an issue, privately with the trainer.

Course Delivery

This course is delivered as a 2 day ‘virtual’ live online training event which is delivered over a 6 hour period on each day, from 9am to 3pm, including breaks. There are optional additional sessions on each day from 3:15pm to 4:15pm where the trainer is available to answer questions on particular method development examples, if desired. The time zone is GMT (UTC). The full schedule of dates is available on the MTS website.

This course is suitable for

Those who have experience of running HPLC methods and now want to learn how to develop new methods or just to understand how they work.
For example:
  • Analytical chemists in industry (the course is not specific to a particular industry)
  • Researchers who need to use HPLC

Included in the course fees

  • Comprehensive course hand-outs - The training book is provided as an electronic copy (pdf) for both live online and classroom based options.
  • Certificate of Attendance
  • Optional post training assessment (accessed in e-MTS, our learning management system) which leads to a Certificate of Training.
  • Access to training materials via e-MTS
  • Post training support – Attendees can contact the trainer with questions that may occur when they apply their learning to real life situations.
Organised by
Mourne Training Services Ltd (MTS) is an independent consultancy specialising in topics relating to analytical laboratories that perform chemical testing of pharmaceuticals and biopharmaceuticals.
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