The early career academics presenting in the next Peptide and Protein Science online series will be Dr Fabian Rehm from Cambridge University and Dr Catherine Hurd from the Manchester Institute of Biotechnology.
Date: 07-06-2024
Time: 1:00-2:00 pm
Venue: an online Zoom lecture
https://eu01web.zoom.us/meeting/register/u5Mrf-qurzMjGN156nxAEH5TD8bOAj7Jyifb#/registration
Dr Catherine Hurd, Wellcome Trust Early Career Fellows, University of Manchester
Title: Expanding the target scope of mRNA display
mRNA display is a powerful technology to screen libraries of more than a trillion cyclic peptides against a protein target, enabling the rapid discovery of high affinity ligands. These cyclic peptides are particularly well suited to challenging protein targets that have been difficult to drug with small molecules. However, target choice can still be limited as screens are typically performed against purified proteins which often demands the use of isolated domains and precludes the use of aggregation-prone targets. We have established a method to perform mRNA display selections in mammalian cell lysates without the need for prior target purification, vastly expanding the potential target scope of mRNA display.
Dr Fabio Rehm – PDRA, MRC Cambridge University
Title: Repurposing a plant peptide cyclase for protein and peptide engineering
Transpeptidases are useful tools for protein and peptide engineering. Asparaginyl endopeptidases that preferentially catalyse transpeptidation rather than proteolysis reactions are found in multiple plant families. Naturally, these enzymes generate head-to-tail cyclised peptides, but their efficient transpeptidation activities have led to these enzymes being repurposed for various protein and peptide modification applications. In this talk, I will focus on our efforts to expand the synthetic scope of this class of enzymes in vitro by tuning the reaction equilibrium, optimising reaction conditions, and designing substrate mimetics. These efforts have enabled us to generate proteins and peptides with various non-canonical linkages and topologies.
For upcoming series, please visit the RSC PPSG website.
If you would like to present in future seminars, please contact one of the organisers.
Louis Luk: lukly@cardiff.ac.uk
Chris Coxon: chris.coxon@ed.ac.uk
Louise Walport: louise.walport@crick.ac.uk
Date: 07-06-2024
Time: 1:00-2:00 pm
Venue: an online Zoom lecture
https://eu01web.zoom.us/meeting/register/u5Mrf-qurzMjGN156nxAEH5TD8bOAj7Jyifb#/registration
Dr Catherine Hurd, Wellcome Trust Early Career Fellows, University of Manchester
Title: Expanding the target scope of mRNA display
mRNA display is a powerful technology to screen libraries of more than a trillion cyclic peptides against a protein target, enabling the rapid discovery of high affinity ligands. These cyclic peptides are particularly well suited to challenging protein targets that have been difficult to drug with small molecules. However, target choice can still be limited as screens are typically performed against purified proteins which often demands the use of isolated domains and precludes the use of aggregation-prone targets. We have established a method to perform mRNA display selections in mammalian cell lysates without the need for prior target purification, vastly expanding the potential target scope of mRNA display.
Dr Fabio Rehm – PDRA, MRC Cambridge University
Title: Repurposing a plant peptide cyclase for protein and peptide engineering
Transpeptidases are useful tools for protein and peptide engineering. Asparaginyl endopeptidases that preferentially catalyse transpeptidation rather than proteolysis reactions are found in multiple plant families. Naturally, these enzymes generate head-to-tail cyclised peptides, but their efficient transpeptidation activities have led to these enzymes being repurposed for various protein and peptide modification applications. In this talk, I will focus on our efforts to expand the synthetic scope of this class of enzymes in vitro by tuning the reaction equilibrium, optimising reaction conditions, and designing substrate mimetics. These efforts have enabled us to generate proteins and peptides with various non-canonical linkages and topologies.
For upcoming series, please visit the RSC PPSG website.
If you would like to present in future seminars, please contact one of the organisers.
Louis Luk: lukly@cardiff.ac.uk
Chris Coxon: chris.coxon@ed.ac.uk
Louise Walport: louise.walport@crick.ac.uk
