The goal of the 20th ISSS symposium will be advancing the knowledge of separation sciences and promoting exchange of new ideas on the academic and the industry level, providing new forum for collaboration and networking. The topics will cover recent advances in the theory, instrumentation and methodology of all types of separation techniques, including new types of particulate and monolithic stationary phases, miniaturized capillary and chip-based fluidic separation media, hyphenated and multi-dimensional separations, method development including chemometric approaches, quality assesment, sample preparation, with emphasis on novel application methods, especialy in food, clinical and environmental analysis.