_tcm18-15276.gif )
Latest News
Interview: Finding answers in blood
27 January 2010
Dana Spence discusses the role of red blood cells in diseases such as diabetes and multiple sclerosis and the importance of a foundation in Chemistry
Interview: A glowing career
14 December 2009
From jellyfish to cancer diagnostics, Roger Tsien discusses the challenges of looking into a cell with Harp Minhas
Imaging tissue growth
09 December 2009
Swiss scientists have investigated how regrowing tissue using scaffolds affects the cell's environment and properties
Contents list for Molecular BioSystems, issue 2, 2010
Front cover
Mol. BioSyst., 2010, 6, 277
DOI: 10.1039/c000131g
Inside front cover
Mol. BioSyst., 2010, 6, 278
DOI: 10.1039/c000132p
Contents
Mol. BioSyst., 2010, 6, 279
DOI: 10.1039/c000133n
Reviews
The use of network analyses for elucidating mechanisms in cardiovascular disease
Diego Diez, Åsa M. Wheelock, Susumu Goto, Jesper Z. Haeggström, Gabrielle Paulsson-Berne, Göran K. Hansson, Ulf Hedin, Anders Gabrielsen and Craig E. Wheelock,
Mol. BioSyst., 2010, 6, 289
DOI: 10.1039/b912078e
Enhanced HTML article available
This paper provides a basic introduction to networks and then examines the use of network analysis to investigate mechanisms in cardiovascular disease by combining results from transcriptomics and literature mining.
Limitations of current therapies to prevent thrombosis: a need for novel strategies
Jean-Etienne Fabre and Mark E. Gurney,
Mol. BioSyst., 2010, 6, 305
DOI: 10.1039/b914375k Enhanced HTML article available
Bleeding limits the benefit of current anti-platelet drugs for preventing heart attacks and stroke. Targeting the EP3 receptor for prostaglandin E2 may avert thrombosis over atherosclerotic plaques without affecting bleeding risk.
PerR vs OhrR: selective peroxide sensing in Bacillus subtilis
Victor Duarte and Jean-Marc Latour,
Mol. BioSyst., 2010, 6, 316
DOI: 10.1039/b915042k Enhanced HTML article available
The specific inducible response of Bacillus subtilis to peroxide stress involves two transcriptional factors, PerR and OhrR. This review points out the latest advances in the peroxide sensing mechanisms for both proteins focusing on biochemical and structural data.
Recent advances and novel approaches in deriving neurons from stem cells
Steven J. Greco and Pranela Rameshwar,
Mol. BioSyst., 2010, 6, 324
DOI: 10.1039/b914822c Enhanced HTML article available
The utilization of stem cells to generate functional neurons has significant clinical implications. Here we highlight recent interdisciplinary approaches used to induce neuronal differentiation from stem cells of varied origin.
Complex genetic regulation of protein glycosylation
Gordan Lauc, Igor Rudan, Harry Campbell and Pauline M. Rudd,
Mol. BioSyst., 2010, 6, 329
DOI: 10.1039/b910377e Enhanced HTML article available
A recently developed high throughput HPLC method for glycan analysis enabled the first genome wide association studies that provide insight into complex network of common genetic polymorphisms that affect human N-glycans.
Communication
Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis
Yoo Seong Choi, Tyler W. Johannes, Michael Simurdiak, Zengyi Shao, Haige Lu and Huimin Zhao,
Mol. BioSyst., 2010, 6, 336
DOI: 10.1039/b923177c Enhanced HTML article available
We report the cloning and heterologous expression of the spectinabilin gene cluster from Streptomyces spectabilis. Unexpectedly, this gene cluster is evolutionarily closer to the aureothin gene cluster than to the spectinabilin gene cluster from Streptomyces orinoci.
Papers
Genome-scale metabolic network analysis and drug targeting of multi-drug resistant pathogen Acinetobacter baumannii AYE
Hyun Uk Kim, Tae Yong Kim and Sang Yup Lee,
Mol. BioSyst., 2010, 6, 339
DOI: 10.1039/b916446d Enhanced HTML article available
Genome-scale metabolic network of multi-drug resistant strain Acinetobacter baumannii AYE was reconstructed for system-wide analysis, and EMFilter was developed to identify essential metabolites and enzymes around them as potential drug targets.
Functional characterization of tlmH in Streptoalloteichus hindustanus E465-94 ATCC 31158 unveiling new insight into tallysomycin biosynthesis and affording a novel bleomycin analog
Meifeng Tao, Liyan Wang, Evelyn Wendt-Pienkowski, Ningning Zhang, Dong Yang, Ute Galm, Jane M. Coughlin, Zhinan Xu and Ben Shen,
Mol. BioSyst., 2010, 6, 349
DOI: 10.1039/b918106g
Isolation and structural elucidation of TLM H-1 and H-2 from the 
tlmH mutant supports the functional assignment of TLMH to catalyze hydroxylation at both C-41 and C-42 for TLM biosynthesis.
A miniaturized screen for inhibitors of Jumonji histone demethylases
Masaaki Sakurai, Nathan R. Rose, Lena Schultz, Amy M. Quinn, Ajit Jadhav, Stanley S. Ng, Udo Oppermann, Christopher J. Schofield and Anton Simeonov,
Mol. BioSyst., 2010, 6, 357
DOI: 10.1039/b912993f Enhanced HTML article available
We present a miniaturized kinetic assay which enables screening of large compound libraries against 2-oxoglutarate- and Fe(II)-dependent N
-methyl lysine demethylases, a major enzyme family implicated in epigenetic signaling.
A strategy to discover inhibitors of Bacillus subtilis surfactin-type phosphopantetheinyl transferase
Adam Yasgar, Timothy L. Foley, Ajit Jadhav, James Inglese, Michael D. Burkart and Anton Simeonov,
Mol. BioSyst., 2010, 6, 365
DOI: 10.1039/b913291k Enhanced HTML article available
Inhibitors of surfactin-type phosphopantetheinyl transferases represent enticing leads for antibiotic development and serve as tools for bacterial metabolism studies. We present a novel kinetic assay utilizing a red-shifted fluorophore–quencher combination which enables screening of compound libraries and is applicable to designing assays for related transferases.
Expansion of the mycobacterial 
PUPylome
Jeramie Watrous, Kristin Burns, Wei-Ting Liu, Anand Patel, Vivian Hook, Vineet Bafna, Clifton E. Barry 3rd, Steve Bark and Pieter C. Dorrestein,
Mol. BioSyst., 2010, 6, 376
DOI: 10.1039/b916104j Enhanced HTML article available
PUPylation is a prokaryotic post-translation modification that, similar to ubiquitin, has been previously implicated in proteasomal degradation. By performing a global proteomic pull-down from Mycobacterium smegmatis 155 protein targets for PUPylation were identifed.
Detection of single nucleotide polymorphisms using a DNA Holliday junction nanoswitch—a high-throughput fluorescence lifetime assay
Colin D. McGuinness, Mira K. Y. Nishimura, David Keszenman-Pereyra, Paul Dickinson, Colin J. Campbell, Till T. Bachmann, Peter Ghazal and Jason Crain,
Mol. BioSyst., 2010, 6, 386
DOI: 10.1039/b913455g Enhanced HTML article available
A DNA sensor capable of detection of single nucleotide polymorphisms in an unlabelled target using a high throughput fluorescence lifetime micro plate reader instrument is reported.
Random network behaviour of protein structures
K. V. Brinda, Saraswathi Vishveshwara and Smitha Vishveshwara,
Mol. BioSyst., 2010, 6, 391
DOI: 10.1039/b903019k Enhanced HTML article available
Side-chain interactions in protein structure networks show random behavior and deviations from this random model have significant biological implications.
Investigation of microsphere-mediated cellular delivery by chemical, microscopic and gene expression analysis
Lois M. Alexander, Salvatore Pernagallo, Alessandra Livigni, Rosario M. Sánchez-Martín, Joshua M. Brickman and Mark Bradley,
Mol. BioSyst., 2010, 6, 399
DOI: 10.1039/b914428e Enhanced HTML article available
The detailed uptake mechanism of microspheres into a range of cell lines was studied. Gene expression profiles of beadfected and untreated cells were used to establish the effect of microsphere uptake at the transcriptional level.
Novel 4
-anilino-podophyllotoxin derivatives: design synthesis and biological evaluation as potent DNA-topoisomerase II poisons and anti-MDR agents
Chunqi Hu, Danqing Xu, Wenting Du, Shijing Qian, Li Wang, Jianshu Lou, Qiaojun He, Bo Yang and Yongzhou Hu,
Mol. BioSyst., 2010, 6, 410
DOI: 10.1039/b912336a Enhanced HTML article available
This paper describes a new series of 4-anilino-podophyllotoxin analogs as topoisomerase II and P-gp-dependent multidrug resistance (MDR) inhibitors. Compound 5n showed a promising potency as a potential drug candidate for anticancer chemotherapy.
Back matter
Mol. BioSyst., 2010, 6, 421
DOI: 10.1039/c000939n
Back cover
Mol. BioSyst., 2010, 6, 423
DOI: 10.1039/c000135j
RSS feed
