The interactive lab primer - thin layer chromatography


The technique of Thin Layer Chromatography (TLC) is normally used as an analytical method to follow the progress of a reaction, to analyse mixtures or to establish conditions for a preparative separation of compounds using column chromatography. The stationary phase (often silica) is coated on plastic or aluminium plates. The mixture is spotted on the plate and solvent is allowed to run up the plate to separate the compounds.

A short animation showing how compounds move up a TLC plate. Flash player is required to view this resource. Use the zoom buttons on your browser to view a larger version of this resource.


This resource is part of the interactive lab primer - lab techniques collection.

See more lab techniques or visit the interactive lab primer home.